Ultraviolet (UV) irradiation is used to disinfect water and food and can be classified as UVA (detected at wavelengths 320–400 nm), UVB (280–320 nm), and UVC (<280 nm). We developed a method for UVA sterilization of equipment with a UVA-light-emitting diode (LED); however, a high rate of fluence was needed to promote pathogen inactivation. The aim of this study was to identify genes associated with UVA sensitivity with the goal of improving UVA-LED-mediated bactericidal activity. We constructed a transposon-mutant library of Vibrio parahaemolyticus and selected six mutants with high sensitivity to UVA irradiation. Genes associated with this phenotype include F-type H⁺-transporting ATPases (atp), as well as those involved in general secretion (gsp), and ubiquinone and terpenoid-quinone biosynthesis (ubi). Gene complementation resulted in decreased sensitivity to UVA-LED. The atp mutants had lower intracellular adenosine triphosphate (ATP) concentrations than the wild-type treatment, with 20 mM L-serine resulting in elevated ATP concentrations and decreased sensitivity to UVA-LED. The gsp mutants exhibited high levels of extracellular protein transport and the ubi mutants exhibited significantly different intracellular concentrations of ubiquinone-8. Taken together, our results suggest that the protein products of the atp, gsp, and ubi genes may regulate sensitivity to UVA irradiation.

中文翻译：

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副溶血弧菌转座子诱变鉴定与紫外线A（UVA）辐射敏感性相关的基因

紫外线（UV）用于消毒水和食品，可分为UVA（在320-400 nm波长处检测到），UVB（280-320 nm）和UVC（<280 nm）。我们开发了一种使用UVA发光二极管（LED）对设备进行UVA灭菌的方法；但是，需要高通量来促进病原体灭活。这项研究的目的是鉴定与UVA敏感性相关的基因，以提高UVA-LED介导的杀菌活性。我们构建了副溶血弧菌的转座子突变体文库，并选择了六个对UVA辐射高度敏感的突变体。与该表型相关的基因包括F型H ⁺转运ATPase（atp），以及与一般分泌有关的基因（gsp），以及泛醌和萜类醌的生物合成（ubi）。基因互补导致对UVA-LED的敏感性降低。的ATP的突变体具有较低的细胞内三磷酸腺苷（ATP）的浓度比野生型治疗，用20mM L-丝氨酸导致升高的ATP浓度和降低到UVA-LED灵敏度。所述GSP突变体显示出高水平的胞外蛋白的运输和UBI突变体表现出泛醌8的显著不同的细胞内浓度。两者合计，我们的结果表明atp，gsp和ubi基因的蛋白质产物可能调节对UVA辐射的敏感性。