Poly(ethylene glycol) (PEG) hydrogels have been extensively used as scaffolds for tissue engineering applications, owing to their biocompatibility, chemical versatility, and tunable mechanical properties. However, their bio‐inert properties require them to be associated with additional functional moieties to interact with cells. To circumvent this need, we propose here to reticulate PEG molecules with poly(L‐lysine) dendrigrafts (DGL) to provide intrinsic cell functionalities to PEG‐based hydrogels. The physico‐chemical characteristics of the resulting hydrogels were studied in regard of the concentration of each component. With increasing amounts of DGL, the cross‐linking time and swelling ratio could be decreased, conversely to mechanical properties, which could be tailored from 7.7 ± 0.7 to 90 ± 28.8 kPa. Furthermore, fibroblasts adhesion, viability, and morphology on hydrogels were then assessed. While cell adhesion significantly increased with the concentration of DGL, cell viability was dependant of the ratio of DGL and PEG. Cell morphology and proliferation; however, appeared mainly related to the overall hydrogel rigidity. To allow cell infiltration and cell growth in 3D, the hydrogels were rendered porous. The biocompatibility of resulting hydrogels of different compositions and porosities was evaluated by 3 week subcutaneous implantations in mice. Hydrogels allowed an extensive cellular infiltration with a mild foreign body reaction, histological evidence of hydrogel degradation, and neovascularization.

中文翻译：

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具有固有生物学特性的多功能赖氨酸树枝状移植物和聚乙二醇水凝胶：体外细胞行为调节和体内生物相容性

聚（乙二醇）（PEG）水凝胶由于其生物相容性、化学多功能性和可调节的机械性能而被广泛用作组织工程应用的支架。然而，它们的生物惰性特性要求它们与额外的功能部分相关联以与细胞相互作用。为了避免这种需求，我们在此建议用聚（L-赖氨酸）树状移植物（DGL）将 PEG 分子网状化，从而为基于 PEG 的水凝胶提供内在的细胞功能。研究了所得水凝胶的物理化学特性，涉及每种成分的浓度。随着 DGL 量的增加，交联时间和溶胀率可以降低，与机械性能相反，机械性能可以从 7.7 ± 0.7 调整到 90 ± 28.8 kPa。此外，成纤维细胞粘附，然后评估水凝胶的活力和形态。虽然细胞粘附随着 DGL 的浓度显着增加，但细胞活力取决于 DGL 和 PEG 的比例。细胞形态和增殖；然而，似乎主要与整体水凝胶刚度有关。为了在 3D 中进行细胞浸润和细胞生长，水凝胶变得多孔。通过在小鼠中进行 3 周皮下植入来评估所得不同成分和孔隙率的水凝胶的生物相容性。水凝胶允许广泛的细胞浸润，并伴有轻微的异物反应、水凝胶降解的组织学证据和新血管形成。细胞活力取决于 DGL 和 PEG 的比例。细胞形态和增殖；然而，似乎主要与整体水凝胶刚度有关。为了在 3D 中进行细胞浸润和细胞生长，水凝胶变得多孔。通过在小鼠中进行 3 周皮下植入来评估所得不同成分和孔隙率的水凝胶的生物相容性。水凝胶允许广泛的细胞浸润，并伴有轻微的异物反应、水凝胶降解的组织学证据和新血管形成。细胞活力取决于 DGL 和 PEG 的比例。细胞形态和增殖；然而，似乎主要与整体水凝胶刚度有关。为了在 3D 中进行细胞浸润和细胞生长，水凝胶变得多孔。通过在小鼠中进行 3 周皮下植入来评估所得不同成分和孔隙率的水凝胶的生物相容性。水凝胶允许广泛的细胞浸润，并伴有轻微的异物反应、水凝胶降解的组织学证据和新血管形成。通过在小鼠中进行 3 周皮下植入来评估所得不同成分和孔隙率的水凝胶的生物相容性。水凝胶允许广泛的细胞浸润，并伴有轻微的异物反应、水凝胶降解的组织学证据和新血管形成。通过在小鼠中进行 3 周皮下植入来评估所得不同成分和孔隙率的水凝胶的生物相容性。水凝胶允许广泛的细胞浸润，并伴有轻微的异物反应、水凝胶降解的组织学证据和新血管形成。