Two Burkholderia gladioli strains isolated from the lungs of cystic fibrosis patients were found to produce unusual lipodepsipeptides containing a unique citrate‐derived fatty acid and a rare dehydro‐β‐alanine residue. The gene cluster responsible for their biosynthesis was identified by bioinformatics and insertional mutagenesis. In‐frame deletions and enzyme activity assays were used to investigate the functions of several proteins encoded by the biosynthetic gene cluster, which was found in the genomes of about 45 % of B. gladioli isolates, suggesting that its metabolic products play an important role in the growth and/or survival of the species. The Chrome Azurol S assay indicated that these metabolites bind ferric iron, which suppresses their production when added to the growth medium. Moreover, a gene encoding a TonB‐dependent ferric‐siderophore receptor is adjacent to the biosynthetic genes, suggesting that these metabolites may function as siderophores in B. gladioli.

中文翻译：

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Bolagladins 的发现和生物合成：来自剑兰伯克霍尔德菌临床分离株的不寻常脂缩肽。

从囊性纤维化患者的肺部分离出两种伯克霍尔德杆菌唐菖蒲菌株，被发现能产生不寻常的脂缩肽，其中含有独特的柠檬酸衍生脂肪酸和罕见的脱氢-β-丙氨酸残基。通过生物信息学和插入诱变鉴定了负责其生物合成的基因簇。使用框内删除和酶活性测定来研究生物合成基因簇编码的几种蛋白质的功能，这些基因簇存在于约 45%唐菖蒲分离株的基因组中，表明其代谢产物在物种的生长和/或生存。Chrome Azurol S 测定表明，这些代谢物与三价铁结合，当添加到生长培养基中时，三价铁会抑制它们的产生。此外，编码 TonB 依赖性铁铁载体受体的基因与生物合成基因相邻，表明这些代谢物可能在剑兰中充当铁载体。