Abstract Irrespective use of chemical pesticides has led, over the last decades, to several problems such as soil, water and food sources pollution, and generation of a selective pressure causing the emergence of pest resistance. Consequently, researchers have been focusing more on the use of biological control as an alternative strategy. Bacillus thuringiensis (Bt) is one of the most widely studied bacteria in industrial biotechnology and commercialized as an environmentally sustainable biopesticide. Therefore, a huge interest has been allocated for research on this bacterium and several scientific studies have been published on the issue. In this review, we tried to evaluate the scientific production over the last thirty years, for the first time, in terms of number and geographical origin, focusing particularly on B. thuringiensis kurstaki (Btk). It is worth emphasizing that the Btk process engineering involving factors affecting growth, sporulation and toxin formation yields by Bt has not been fully investigated in previous reviews. To this end, the second section of this review provided an updated survey about these conditions, such as nutritional requirements, culture media and fermentation technologies. Relevant information was collected in comparative tables that could be very useful for the scientific community interested in Btk-based biopesticides.

中文翻译：

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苏云金芽孢杆菌生产生物农药的研究进展。kurstaki 自 1990 年以来：专注于生物工艺参数

摘要 在过去的几十年里，不考虑使用化学杀虫剂已经导致了一些问题，例如土壤、水和食物来源的污染，以及导致害虫抗性出现的选择压力的产生。因此，研究人员更多地关注使用生物控制作为替代策略。苏云金芽孢杆菌 (Bt) 是工业生物技术中研究最广泛的细菌之一，并作为一种环境可持续的生物农药商业化。因此，人们对这种细菌的研究产生了巨大的兴趣，并已就此问题发表了几项科学研究。在这篇综述中，我们首次尝试从数量和地理起源方面评估过去三十年的科学成果，特别关注 B. 苏云金库斯塔基 (Btk)。值得强调的是，涉及影响 Bt 生长、孢子形成和毒素形成产量的因素的 Btk 工艺工程在以前的评论中尚未得到充分研究。为此，本综述的第二部分提供了有关这些条件的最新调查，例如营养需求、培养基和发酵技术。在比较表中收集了相关信息，这些信息对于对基于 Btk 的生物农药感兴趣的科学界非常有用。例如营养需求、培养基和发酵技术。在比较表中收集了相关信息，这些信息对于对基于 Btk 的生物农药感兴趣的科学界非常有用。例如营养需求、培养基和发酵技术。在比较表中收集了相关信息，这些信息对于对基于 Btk 的生物农药感兴趣的科学界非常有用。