Peppermint oil (PO) has widely been reported for their nutritional and biomedical properties. Essential oil (EO) has several properties, usage restricted due to environmental condition, polymer based nanoencapsulation of EOs is developed as one of the promising technique to address this limitation. In this current study emphasizes on developing Peppermint oil (PO) encapsulated chitosan nanoparticles (CS NPs) by ionic gelation method in order to improving its insecticidal potential effective management of two stored grain pest. The nano-encapsulated PO (CS/PO NPs) was confirmed by UV-Vis spectrophotometry and X-ray diffraction (XRD) techniques. The physicochemical characterization of CS/PO NPs revealed <563.3 nm size, Zeta potential of -12.12 mV, encapsulation efficiency >64% and loading capacity >12.31% respectively. Developed CS/PO NPs demonstrated the toxicity studies have significantly efficacy against both stored product pest compared to control. In-vivo percent inhibition of AChE activity ranged between S. oryzae (52.43% and 37.71% and and T. castaneum (37.80% and 31.29). Overall, these results highlighted that polymer chitosan nanoparticle encapsulated with essential oil is a promising novel design that could be promoted in stored food pest management schedule for T. castaneum and S. oryzae.

中文翻译：

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壳聚糖纳米颗粒封装薄荷精油的开发：表征和对储粮害虫控制的生物学功效

薄荷油 (PO) 因其营养和生物医学特性而被广泛报道。精油 (EO) 具有多种特性，使用受到环境条件的限制，基于聚合物的 EO 纳米封装被开发为解决这一限制的有前途的技术之一。在目前的研究中，强调通过离子凝胶法开发薄荷油 (PO) 封装的壳聚糖纳米颗粒 (CS NPs)，以提高其对两种储粮害虫的杀虫潜力有效管理。通过紫外可见分光光度法和 X 射线衍射 (XRD) 技术证实了纳米封装的 PO (CS/PO NPs)。CS/PO NPs 的理化特性分别显示 <563.3 nm 的尺寸、-12.12 mV 的 Zeta 电位、封装效率 >64% 和负载能力 >12.31%。开发的 CS/PO NP 表明，与对照相比，毒性研究对两种储存产品害虫都具有显着功效。AChE 活性的体内抑制百分比介于 S. oryzae（52.43% 和 37.71% 和 T. castaneum（37.80% 和 31.29）之间。总体而言，这些结果强调了用精油包裹的聚合物壳聚糖纳米颗粒是一种很有前途的新设计可以在 T. castaneum 和 S. oryzae 的储藏食品害虫管理计划中推广。