In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.

中文翻译：

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一种改进的丁香假单胞菌的缀合方法。

为了实现丁香假单胞菌的植物致病菌株基因组的饱和转座子诱变，我们需要提高质粒的缀合频率。供体和受体细胞的生长阶段的操纵允许所需的频率增加，并促进其他顽固菌株的结合。