Abstract Mammalian cell cultures are widely used in the biopharmaceutical industry to produce monoclonal antibodies, vaccines, and growth factors, etc. Cell death is an essential biological process for physiological growth and development, but it is a major problem in biopharmaceutical production in bio-industry. Cell death within bioreactor occurs due to various intracellular and extracellular stresses. These stresses negatively affect the culture longevity, overall product quality, and yield. Among all cell death types, apoptosis accounts for most of the cellular death in the bioreactor. The implementation and development of various strategies to prevent the cellular death are crucial for robust bioprocess development. Cell death during culture can be prevented or inhibited by supplementing media with specific chemicals, synthetic inhibitors, and genetic cell engineering approaches. In this review, we classified and described different types of cell death and their molecular mechanisms and summarized the cell death inhibition approaches implemented to inhibit cell death for various applications.

中文翻译：

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培养中的细胞死亡：分子机制、检测和抑制策略

摘要 哺乳动物细胞培养广泛应用于生物制药行业，生产单克隆抗体、疫苗、生长因子等。细胞死亡是生理生长发育必不可少的生物过程，是生物制药生产中的一大难题。 . 由于各种细胞内和细胞外应激，生物反应器内的细胞死亡发生。这些压力会对培养寿命、整体产品质量和产量产生负面影响。在所有细胞死亡类型中，细胞凋亡占生物反应器中细胞死亡的大部分。防止细胞死亡的各种策略的实施和开发对于稳健的生物过程开发至关重要。可以通过向培养基补充特定化学品、合成抑制剂、和基因细胞工程方法。在这篇综述中，我们对不同类型的细胞死亡及其分子机制进行了分类和描述，并总结了用于抑制各种应用的细胞死亡的细胞死亡抑制方法。