Gold Nanoparticles (AuNPs) are a class of promising nanomaterial for biomedical applications ranging from bioimaging, drug delivery to phototherapy because of their biocompatibility, easily tunable size and shape, and versatile surface modifications. In recent years, the rapid development of AuNPs in nanomedicine has made it imperative to seek fundamental understanding on their nano-biointeractions to minimize adverse effects and improve targeting/imaging efficiency. In this review, we summarize the different pathways of NPs-biomembrane interactions with a focus on AuNPs, follow by an analysis on how the physiochemical properties (size, surface charge, shape, surface ligands, and hydrophobicity etc.) of AuNPs can be involved in the mechanisms of cellular uptake. Finally, some recent advances on simulation modelling of AuNPs-biomembrane interactions and a brief outlook in the field are discussed.

金纳米粒子（AuNPs）由于其生物相容性，易于调节的尺寸和形状以及通用的表面修饰，因此是一类有前途的纳米材料，可用于生物医学，药物输送到光疗等生物医学应用。近年来，AuNP在纳米药物中的快速发展已经迫切需要对它们的纳米生物相互作用寻求基本的了解，以最大程度地减少不利影响并提高靶向/成像效率。在这篇综述中，我们总结了以AuNPs为重点的NPs-生物膜相互作用的不同途径，然后分析了如何涉及AuNPs的理化性质（大小，表面电荷，形状，表面配体和疏水性等）。在细胞摄取机制中。最后，