Cellulases are enzymes required for the production of second-generation ethanol (E2G) via biochemical route. The current paper reports the development of an apparatus for solid-liquid extraction of cellulases from solid-state fermentation (SSF) carried out in a packed bed bioreactor (PBB), operated as batch and as semicontinuous. The case study was the cultivation of Myceliophthora thermophila I-1D3b on sugarcane bagasse (SCB) and wheat bran (WB) (7: 3 w/w). The current work integrates the PBB to the first downstream step for recovering the enzymes produced by SSF. The substrate was inoculated and packed into the modules that composed the PBB. The fermentation occurred at 45 °C and air was supplied with flow rate of 350 L/h. At the end of the cultivations, each module was placed in an extraction column, a dynamic closed system in which distilled water was circulated and made to percolate the cultivated material. Variables tested were volume of water per mass of substrate, water flow rate and time of percolation. Higher contact time (120 min) and higher flow rate (2.4m3/h) allowed us to recover up to 85% of total enzyme activity by percolation. Lower volume (20mL/g) provides higher titer extract. The apparatus showed to be promising for SSF downstream, mainly for semicontinuous operation of PPBs.

中文翻译：

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在填充床生物反应器中固液提取真菌固态培养中的纤维素酶

纤维素酶是通过生化途径生产第二代乙醇 (E2G) 所需的酶。目前的论文报道了一种用于从固态发酵 (SSF) 中固液提取纤维素酶的装置的开发，该装置在填充床生物反应器 (PBB) 中进行，以分批和半连续方式运行。案例研究是在甘蔗渣 (SCB) 和麦麸 (WB) (7: 3 w/w) 上培养嗜热毁丝霉 I-1D3b。目前的工作将 PBB 整合到第一个下游步骤中，以回收 SSF 产生的酶。将基材接种并装入组成 PBB 的模块中。发酵在 45 °C 下进行，空气以 350 L/h 的流速供应。在培养结束时，将每个模块放置在提取柱中，一种动态封闭系统，其中蒸馏水循环并渗透培养材料。测试的变量是每质量基材的水体积、水流速和渗透时间。更长的接触时间（120 分钟）和更高的流速（2.4 立方米/小时）使我们能够通过渗透回收高达 85% 的总酶活性。较低的体积 (20mL/g) 提供更高滴度的提取物。该装置显示出有希望用于 SSF 下游，主要用于 PPB 的半连续操作。