Ubiquitination is a post-translational modification involved in diverse cellular processes in eukaryotes. Among three kinds of enzymes (E1, E2, and E3) involved in ubiquitination, the E3 ubiquitin ligase family possesses a large number of members (up to 1400) and serves as a major determinant for substrate specificity. As a type of E3 ligase, cullin-RING E3 ligase (CRL) forms multi-subunit complexes classified into three types, CRL1, CRL3, and CRL4, in Arabidopsis. Of E3 enzymes, the CRL1 complex, also known as the SCF complex, is the largest family and has been most fully characterized so far. Studies of the biological role of CRL1 have mainly concentrated on the functional elucidation of F-box proteins, serving as substrate receptors of CRL1. F-box proteins possess an N-terminal F-box motif consisting of 40–50 amino acids and a variable C-terminal substrate-interacting domain. An F-box domain and a substrate-interacting domain are responsible for the interaction of SKP1 as an adaptor of CRL1 and the target protein(s) to be ubiquitinated, respectively. There are many F-box proteins (e.g., ~ 690 members in Arabidopsis), and CRL1 complexes generated from diverse F-box proteins are widely involved in most events occurring in plant cells. In this study, we review the current knowledge of and future prospects for CRL1 complexes with our main focus on F-box proteins, which function in developmental processes, hormone responses, and biotic/abiotic stress responses in Arabidopsis. Our review will enable a broadening of the understanding of the functional relationships between the CRL1-mediated ubiquitination process and Arabidopsis cellular responses.

泛素化是真核生物中涉及多种细胞过程的翻译后修饰。在涉及泛素化的三种酶（E1，E2和E3）中，E3泛素连接酶家族拥有大量成员（最多1400个），并且是底物特异性的主要决定因素。作为E3连接酶的一种，cullin-ring E3连接酶（CRL）在拟南芥中形成多亚基复合物，分为三种类型，即CRL1，CRL3和CRL4。。在E3酶中，CRL1复合物（也称为SCF复合物）是最大的家族，迄今已得到最充分的表征。CRL1的生物学作用的研究主要集中在F-box蛋白的功能阐明上，该蛋白是CRL1的底物受体。F-box蛋白具有由40-50个氨基酸组成的N端F-box基序和一个可变的C端底物相互作用域。F-box结构域和底物相互作用结构域分别负责作为CRL1衔接子的SKP1与待泛素化的靶蛋白的相互作用。F-box蛋白很多（例如，拟南芥中约有690个成员）），由多种F-box蛋白产生的CRL1复合物广泛参与植物细胞中发生的大多数事件。在这项研究中，我们回顾了CRL1复合物的当前知识和未来前景，我们主要关注F-box蛋白，该蛋白在拟南芥中的发育过程，激素反应以及生物/非生物胁迫反应中发挥作用。我们的审查将能够拓宽对CRL1介导的泛素化过程与拟南芥细胞反应之间功能关系的理解。