Journal of Environmental Health Science and Engineering ( IF 3.4 ) Pub Date : 2020-08-11 , DOI: 10.1007/s40201-020-00519-3 Maryam Didari 1 , Maryam Bagheri 1 , Mohammad Ali Amoozegar 1 , Saied Bouzari 2 , Hamid Babavalian 3 , Hamid Tebyanian 4 , Mehdi Hassanshahian 5 , Antonio Ventosa 6
Purpose
In this study, the culturable halophilic and halotolerant bacterial diversity was determined in Aran-Bidgol as a thalassohaline seasonal hypersaline lake in Iran.
Methods
Thirty water, soil, sediments, coastal mud, multi-color brines and salt crystals samples were extracted and cultured using different media and incubation conditions. Totally 958 isolates were obtained and 87 isolates were selected for further studies, based on morphological, physiological and biochemical tests, representing different morphotypes.
Results
Based on 16S rRNA gene sequence analyses, the isolates exhibited 94.6–100% sequence similarity to the closest known species of the genera Bacillus, Halomonas, Oceanobacillus, Salinicoccus, Thalassobacillus, Ornithinibacillus, Halobacillus, Salicola, Virgibacillus, Aerococcus, Arthrobacter, Idiomarina, Paraliobacillus, Staphylococcus, Acinetobacter, Aneurinibacillus, Brevibacillus, Brevundimonas, Chromohalobacter, Gracilibacillus, Jeotgalicoccus, Kocuria, Marinilactibacillus, Marinobacter, Microbacterium, Paenibacillus, Paracoccus, Piscibacillus, Pseudomonas and Sediminibacillus and also, comparison of ARDRA patterns among the sequenced strains, using AluI, Bst UI and Hpa II enzymes showed that these patterns are in accordance with the phylogenetic position of these strains.
Conclusion
The PCR-RFLP analyses suggested that ARDRA possess a functional potential for distinguishing halophilic bacteria to be used for further studies in elementary steps of isolation to reduce the tedious duplication of isolates.
中文翻译:
最大的季节性高盐湖(Aran-Bidgol-Iran)中嗜盐和耐盐细菌的多样性
目的
在这项研究中,在 Aran-Bidgol 中确定了可培养的嗜盐和耐盐细菌多样性,作为伊朗的一个海水淡化季节性高盐湖。
方法
使用不同的培养基和培养条件提取和培养了 30 个水、土壤、沉积物、海岸泥浆、多色盐水和盐晶体样品。经形态学、生理生化试验,共获得958株,选择87株进行进一步研究,代表不同的形态型。
结果
基于 16S rRNA 基因序列分析,分离株与芽孢杆菌属、盐单胞菌属、海洋杆菌属、盐球菌属、海洋杆菌属、鸟氨酸杆菌属、盐杆菌属、水杨属、弧菌属、气球菌属、节杆菌属、Idiomarina属、副杆菌属的已知物种具有 94.6-100% 的序列相似性,葡萄球菌, 不动杆菌, 无神经芽孢杆菌,短杆菌属,短杆菌属,嗜铬杆菌属, Gracilibacillus , Jeotgalicoccus , Kocuria , Marinilactibacillus , Marinobacter , Microbacterium , Paenibacillus , Paracoccus , Piscibacillus , Pseudomonas和Sediminibacillus以及测序菌株之间的 ARDRA 模式比较, 使用Alu I, Bst UI 和Hpa II 酶表明这些模式在根据这些菌株的系统发育位置。
结论
PCR-RFLP 分析表明,ARDRA 具有区分嗜盐细菌的功能潜力,可用于进一步研究分离的基本步骤,以减少分离株的繁琐重复。