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A simple method for isolation and culture of primary hepatocytes from Salvelinus leucomaenis (White-spotted Charr).
Cytotechnology ( IF 2.2 ) Pub Date : 2020-08-10 , DOI: 10.1007/s10616-020-00415-6
Katsuya Iuchi 1 , Yasushi Arai 2 , Kazuki Sasaki 1 , Naoe Sato 2 , Chikako Yokoyama 3 , Toshiro Saruwatari 4 , Hisashi Hisatomi 1
Affiliation  

White-spotted charr (Salvelinus leucomaenis, S. I.) is an anadromous cold water-adapted fish, distributed in the Far East. We have previously reported the complete mitochondrial DNA sequences of white-spotted chars (S. l. imbrius and S. l. pluvius) in Japan. In general, fish hepatocytes are useful for cellular and biochemical studies of fish. In this study, we isolated hepatocytes from the liver of white-spotted charr and used basic methods, such as enzyme digestion and low centrifugation, to analyze the molecular mechanisms involved in specific cellular responses. The isolated hepatocytes could be cultured at 5–20 °C but not 37 °C. The morphology of hepatocytes was altered in a temperature-dependent manner. The properties of hepatocyte were similar to those of living fish. Moreover, the proliferation rate and damage of isolated hepatocytes depended on the concentration of fetal bovine serum in the culture medium. Taken together, this study demonstrates that this simple method for isolation and culture of hepatocytes from white-spotted charr may be useful for other biochemical and cellular studies.



中文翻译:

一种从 Salvelinus leucomaenis (White-spotted Charr) 中分离和培养原代肝细胞的简单方法。

白点夏尔(远东红点鲑小号)是溯河冷水适应鱼类,分布在远东地区。我们之前已经报道了白点炭(S. l. imbriusS. l. pluvius )的完整线粒体 DNA 序列) 在日本。一般来说,鱼肝细胞可用于鱼的细胞和生化研究。在这项研究中,我们从白点炭疽的肝脏中分离出肝细胞,并使用酶消化和低速离心等基本方法来分析参与特定细胞反应的分子机制。分离的肝细胞可以在 5-20°C 下培养,但不能在 37°C 下培养。肝细胞的形态以温度依赖性方式改变。肝细胞的特性与活鱼相似。此外,分离肝细胞的增殖率和损伤取决于培养基中胎牛血清的浓度。综合起来,

更新日期:2020-08-11
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