The enormous availability of lignocellulosic biomass makes it a potential renewable feedstock for the continuous supply of second-generation bioethanol. The present study focuses on the exploitation of jute biomass left over after fiber extraction for bioethanol production by subjecting it to physical and chemical pretreatments (alkali, acid, ammonia fiber expansion, steam) followed by enzymatic saccharification and fermentation. The compositional analysis of jute biomass indicated that it contained cellulose (42.52 ± 5.54%), hemicellulose (12.24 ± 0.06%), lignin (31.58 ± 3.67%), and extractives (6.21 ± 0.42%). The biomass was subjected to different pretreatments and the structural and chemical changes along with crystallinity of cellulose were examined through scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction, respectively. Enzymatic saccharification of pretreated biomass revealed that among all the pretreatment methods, alkali (2% NaOH) treated substrate produced significantly higher amount of reducing sugar (19.51 g/L) compared with other pretreated biomass after 72 h of hydrolysis. The fermentation of the reducing sugars released during saccharification was carried out by a thermotolerant yeast Saccharomyces cerevisiae JRC6 resulting in 7.55 g/L of ethanol within 72 h of fermentation with 77.73% fermentation efficiency. Furthermore, lignin was aslo recovered from the spent liquor obtained after alkali pretreatment of the substrate and the remnant wash was analyzed by LC-MS for the presence of valuable platform chemicals. This study for the first time illustrates the potential of jute sticks as a feedstock for second-generation bioethanol.

木质纤维素生物质的巨大可用性使其成为连续供应第二代生物乙醇的潜在可再生原料。本研究的重点是对纤维提取后残留的黄麻生物质进行开发，以对其进行生物和物理预处理（碱，酸，氨纤维膨胀，蒸汽），然后进行酶促糖化和发酵，以生产生物乙醇。黄麻生物质的组成分析表明，它含有纤维素（42.52±5.54％），半纤维素（12.24±0.06％），木质素（31.58±3.67％）和提取物（6.21±0.42％）。对生物质进行了不同的预处理，并通过扫描电子显微镜（SEM），傅里叶变换红外光谱（FTIR），和X射线衍射。预处理生物质的酶促糖化表明，在所有预处理方法中，经过72 h水解后，碱（2％NaOH）处理的底物与其他预处理生物质相比产生的还原糖量（19.51 g / L）明显更高。糖化过程中释放的还原糖的发酵是通过耐热酵母进行的酿酒酵母JRC6在发酵72小时内产生7.55 g / L乙醇，发酵效率为77.73％。此外，从对底物进行碱预处理后获得的废液中也回收了木质素，并通过LC-MS分析了残留洗涤液中有价值的平台化学品的存在。这项研究首次说明了黄麻棒作为第二代生物乙醇原料的潜力。