The application value of Pseudomonas protegens in agriculture has been widely studied, but the manufacturing processes used to produce P. protegens limits its usefulness as a biological control agent because of its poor shelf life due to low stress resistance during processing. We combined hyperosmotic cultivation and fluidized-bed drying to prepare much more stable P. protegens preparations. During drying and cryogenic storage, the viability of hyperosmotic cells was significantly improved compared to that of cells prepared by previous methods. Addition of skim milk provided an excellent protective effect for both hyperosmotic and standard cells. In two field trials, when the concentration of the P. protegens preparation was 0.333% w/v, the biocontrol efficacy against tomato bacterial wilt was more than 80%, which was significantly higher than that of the control provided by the antibiotics zhongshengmycin and streptomycin sulfate. The results show that P. protegens products can be successfully prepared by combination of hyperosmotic cultivation and fluidized-bed drying.

假单胞菌蛋白在农业中的应用价值已得到广泛研究，但生产P的生产工艺却很普遍。由于加工过程中的低抗应力性，其保质期很短，因此蛋白原限制了其作为生物防治剂的用途。我们将高渗栽培与流化床干燥相结合，以制备更稳定的磷。蛋白质制剂。在干燥和低温储存期间，与通过先前方法制备的细胞相比，高渗细胞的生存能力显着提高。添加脱脂乳对高渗细胞和标准细胞均具有出色的保护作用。在两次野外试验中，当P。蛋白质制备量为0.333％w / v，对番茄青枯病的生防效力超过80％，明显高于抗生素中生霉素和硫酸链霉素提供的防治效果。结果表明，P。通过高渗培养和流化床干燥相结合，可以成功制备出蛋白质产品。