Tricholepis roylei Hook. f. is a bushy annual shrub of the Asteraceae family and point endemic species of the Western Himalaya. T. roylei is a critically endangered species and factors like poor seed germination and habitat destruction are further rendering the species towards extinction. Therefore, the present investigation was designed to document the seed germination potential of the species and to establish a reproducible in vitro propagation and mass multiplication protocol for the ex situ conservation of T. roylei germplasm. Seeds of T. roylei were collected from Sangdha, Himachal Pradesh, India, and were sown in different substrates under open and controlled conditions. Though the overall seed germination potential of the species was reported to be very low and maximum 45% germination was observed in cocopeat substrate after 26 days of incubation. Half-strength Murashige and Skoog (MS) medium enriched with 6-benzylaminopurine (4.4 μM l−1) and naphthalene acetic acid (1.0 μM l-1) was observed to be the optimum medium for shoot induction in shoot tip explants of T. roylei. Maximum 98.89% shoot formation was observed with 28.42 shoots per culture and 4.4-cm shoot length, respectively. The healthy shoots (4.0 cm) were transferred onto rooting media (1/4, 1/2 and full MS) and roots were developed after 8 weeks of incubation in the half-strength MS medium. Half MS augmented with 4.9 μM l−1 indole butyric acid was observed to be optimum for the root development and an average of 10.2 roots per shoot with 4.0-cm length was obtained. Rooted plantlets were successfully acclimatized under greenhouse conditions and subsequently established in the field, with a recorded survival rate of 90%. The plants acclimatized to the open environment were also planted in the wild under the habitat rehabilitation and species recovery programme. The seed germination study envisages that the seed germination potential of the species is very poor and might be one of the probable factors responsible for the shrinkage of T. roylei population in the wild. The standardized micropropagation protocol can be helpful for the ex situ conservation of germplasm and rehabilitation of species in the wild. Moreover, the study could be helpful in elucidating the phytochemical and molecular analysis of species.

中文翻译：

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Tricholepis roylei钩的微繁殖。F.-西喜马拉雅山的一种点特有物种。

Tricholepis roylei钩。F。是菊苣科和喜马拉雅西部特有种的灌木丛。T. roylei是一种极度濒危的物种，诸如种子发芽差和栖息地破坏等因素进一步加剧了该物种的灭绝。因此，本研究旨在记录该物种的种子发芽潜力，并建立可再生的体外繁殖和质量繁殖方案，以用于罗勒叶种质的非原生境保存。T. roylei的种子采自印度喜马al尔邦的桑达（Sangdha），并在开放和受控的条件下播种在不同的基质中。虽然据报道该物种的整体种子发芽潜力非常低，并且在孵育26天后在copcopeat基质中观察到最大45％的发芽。观察到富含6-苄基氨基嘌呤（4.4μM-1）和萘乙酸（1.0μM-1）的半强度Murashige和Skoog（MS）培养基是诱导T芽外植体芽诱导的最佳培养基。 roylei。观察到最大98.89％的芽形成，每种培养物分别有28.42个芽和4.4cm的芽长。将健康芽（4.0厘米）转移到生根培养基（1 / 4、1 / 2和完全MS）上，并在半强度MS培养基中孵育8周后，将根发育。观察到用4.9μM1-1吲哚丁酸增强的半数MS最适合根部发育，平均每根芽有10.2根，为4。获得0厘米的长度。根系小植株在温室条件下成功地驯化，随后在田间建立，记录的成活率为90％。在生境恢复和物种恢复计划下，还适应野外环境的植物也已在野外种植。种子发芽研究认为，该物种的种子发芽潜力非常低，并且可能是造成野生罗勒叶草种群减少的可能因素之一。标准化的微繁殖方案可以帮助野生种的异质保存和物种恢复。此外，该研究可能有助于阐明物种的植物化学和分子分析。根系小植株在温室条件下成功地驯化，随后在田间建立，记录的成活率为90％。在生境恢复和物种恢复计划下，还适应野外环境的植物也已在野外种植。种子发芽研究认为，该物种的种子发芽潜力非常低，并且可能是造成野生罗勒叶草种群减少的可能因素之一。标准化的微繁殖方案可以帮助野生种的异质保存和物种恢复。此外，该研究可能有助于阐明物种的植物化学和分子分析。根系小植株在温室条件下成功地驯化，随后在田间建立，记录的成活率为90％。在生境恢复和物种恢复计划下，还适应野外环境的植物也已在野外种植。种子发芽研究认为，该物种的种子发芽潜力非常低，并且可能是造成野生罗勒叶草种群减少的可能因素之一。标准化的微繁殖方案可以帮助野生种的异质保存和物种恢复。此外，该研究可能有助于阐明物种的植物化学和分子分析。在生境恢复和物种恢复计划下，还适应了开放环境的植物在野外种植。种子发芽研究认为，该物种的种子发芽潜力非常低，并且可能是造成野生罗勒叶草种群减少的可能因素之一。标准化的微繁殖方案可以帮助野生种的异质保存和物种恢复。此外，该研究可能有助于阐明物种的植物化学和分子分析。在生境恢复和物种恢复计划下，还适应野外环境的植物也已在野外种植。种子发芽研究认为，该物种的种子发芽潜力非常低，并且可能是造成野生罗勒叶草种群减少的可能因素之一。标准化的微繁殖方案可以帮助野生种的异质保存和物种恢复。此外，该研究可能有助于阐明物种的植物化学和分子分析。种子发芽研究认为，该物种的种子发芽潜力非常低，并且可能是造成野生罗勒叶草种群减少的可能因素之一。标准化的微繁殖方案可以帮助野生种的异质保存和物种恢复。此外，该研究可能有助于阐明物种的植物化学和分子分析。种子发芽研究认为，该物种的种子发芽潜力非常低，并且可能是造成野生罗勒叶草种群减少的可能因素之一。标准化的微繁殖方案可以帮助野生种的异质保存和物种恢复。此外，该研究可能有助于阐明物种的植物化学和分子分析。