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Polylactic acid-based electrospun fiber and hyaluronic acid-valsartan hydrogel scaffold for chronic wound healing
Beni-Suef University Journal of Basic and Applied Sciences Pub Date : 2020-08-10 , DOI: 10.1186/s43088-020-00057-9
Margaret O. Ilomuanya , Prosper S. Okafor , Joyce N. Amajuoyi , John C. Onyejekwe , Omotunde O. Okubanjo , Samson O. Adeosun , Boladale O. Silva

In this study, the chronic wound healing ability of PLA-based electrospun nanofibers loaded with hyaluronic acid, valsartan, and ascorbic acid is explored. PLA-based scaffolds were fabricated by electrospinning, followed by loading the scaffolds with different concentrations of hyaluronic acid, valsartan, and ascorbic acid hydrogels. The produced formulations were characterized by scanning electron microscopy imaging (SEM), tensile strength testing, Fourier-transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC). An in vitro drug release study was conducted to monitor the release of valsartan from the different formulations. This was followed by exploring the wound healing effects of the scaffolds in alloxan-induced diabetic rats and comparing the wound healing effects with positive and negative controls. The average diameter of the fibers was in the range of 300 to 490 nm with high porosity in the range of 63.90 to 79.44%, offering a large surface area-to-volume ratio, enhanced drug solubility, oxygen permeability, and fluid uptake. The presence of valsartan significantly impacted on the re-epithelization rate. Percentage re-epithelization rate was 31.2% ± 1.77% in the absence of treatment. Histologic section of tissue showed skin with underlying loose fibro-collagenous stroma (dermis) containing sebaceous glands and hair follicles for animals treated with VA, VB, VC, and VD. All the scaffolds reduced the number of inflammatory cell infiltrates at the wound site compared to the no treatment and conventionally treated groups. Conventional antibiotic treatment and VD (electrospun biomimetic scaffolds containing ascorbic acid) had % re-epithelization rates of 59.45% ± 1.69% and 62.01% ± 1.68% which were significantly lower than the PLA/HA-valsartan hydrogel scaffolds with VB having the highest % re-epithelization rate of 85.5% ± 1.7% (Figure 4B & 5C). This study explored the use of biomimetic polylactic acid-based electrospun fiber and HA-valsartan hydrogel scaffold incorporating topical angiotensin receptor blockers to successfully accelerate wound healing. The novel PLA-based electrospun fibers loaded with hyaluronic acid-valsartan hydrogels were stable and possessed proven diabetic wound healing property. This was as a result of the known biomimetic effect of the fibers and increased re-epithelization facilitated by the hydrogels containing valsartan.

中文翻译:

聚乳酸基电纺纤维和透明质酸缬沙坦水凝胶支架用于慢性伤口愈合

在这项研究中,探索了负载透明质酸、缬沙坦和抗坏血酸的 PLA 基电纺纳米纤维的慢性伤口愈合能力。基于 PLA 的支架通过静电纺丝制造,然后在支架上加载不同浓度的透明质酸、缬沙坦和抗坏血酸水凝胶。通过扫描电子显微镜成像 (SEM)、拉伸强度测试、傅里叶变换红外光谱 (FTIR) 和差示扫描量热法 (DSC) 对生产的配方进行表征。进行体外药物释放研究以监测缬沙坦从不同制剂中的释放。随后探索了支架在四氧嘧啶诱导的糖尿病大鼠中的伤口愈合效果,并将伤口愈合效果与阳性和阴性对照进行了比较。纤维的平均直径在 300 到 490 nm 范围内,孔隙率在 63.90 到 79.44% 范围内,提供了大的表面积与体积比,增强了药物溶解性、透氧性和液体吸收。缬沙坦的存在显着影响上皮再生率。在没有治疗的情况下,再上皮化率为 31.2% ± 1.77%。对于用 VA、VB、VC 和 VD 治疗的动物,组织的组织学切片显示皮肤具有底层松散的纤维胶原基质(真皮),其中含有皮脂腺和毛囊。与未治疗组和常规治疗组相比,所有支架都减少了伤口部位炎症细胞浸润的数量。常规抗生素治疗和 VD(含有抗坏血酸的电纺仿生支架)的再上皮化率为 59.45% ± 1.69% 和 62.01% ± 1.68%,明显低于 PLA/HA-缬沙坦水凝胶支架,其中 VB 的百分比最高再上皮化率为 85.5% ± 1.7%(图 4B 和 5C)。本研究探索了使用仿生聚乳酸基电纺纤维和 HA-缬沙坦水凝胶支架结合局部血管紧张素受体阻滞剂成功加速伤口愈合。装载有透明质酸-缬沙坦水凝胶的新型 PLA 基电纺纤维是稳定的,并具有经证实的糖尿病伤口愈合特性。这是由于纤维的已知仿生效应和含有缬沙坦的水凝胶促进的再上皮化增加的结果。45% ± 1.69% 和 62.01% ± 1.68%,明显低于 PLA/HA-缬沙坦水凝胶支架,VB 的再上皮化率最高,为 85.5% ± 1.7%(图 4B 和 5C)。本研究探索了使用仿生聚乳酸基电纺纤维和 HA-缬沙坦水凝胶支架结合局部血管紧张素受体阻滞剂成功加速伤口愈合。装载有透明质酸-缬沙坦水凝胶的新型 PLA 基电纺纤维是稳定的,并具有经证实的糖尿病伤口愈合特性。这是由于纤维的已知仿生效应和含有缬沙坦的水凝胶促进的再上皮化增加的结果。45% ± 1.69% 和 62.01% ± 1.68%,明显低于 PLA/HA-缬沙坦水凝胶支架,VB 的再上皮化率最高,为 85.5% ± 1.7%(图 4B 和 5C)。本研究探索了使用仿生聚乳酸基电纺纤维和 HA-缬沙坦水凝胶支架结合局部血管紧张素受体阻滞剂成功加速伤口愈合。装载有透明质酸-缬沙坦水凝胶的新型 PLA 基电纺纤维是稳定的,并具有经证实的糖尿病伤口愈合特性。这是由于纤维的已知仿生效应和含有缬沙坦的水凝胶促进的再上皮化增加的结果。68%,显着低于 PLA/HA-缬沙坦水凝胶支架,VB 的再上皮化率最高,为 85.5% ± 1.7%(图 4B 和 5C)。本研究探索了使用仿生聚乳酸基电纺纤维和 HA-缬沙坦水凝胶支架结合局部血管紧张素受体阻滞剂成功加速伤口愈合。装载有透明质酸-缬沙坦水凝胶的新型 PLA 基电纺纤维是稳定的,并具有经证实的糖尿病伤口愈合特性。这是由于纤维的已知仿生效应和含有缬沙坦的水凝胶促进的再上皮化增加的结果。68%,显着低于 PLA/HA-缬沙坦水凝胶支架,VB 的再上皮化率最高,为 85.5% ± 1.7%(图 4B 和 5C)。本研究探索了使用仿生聚乳酸基电纺纤维和 HA-缬沙坦水凝胶支架结合局部血管紧张素受体阻滞剂成功加速伤口愈合。装载有透明质酸-缬沙坦水凝胶的新型 PLA 基电纺纤维是稳定的,并具有经证实的糖尿病伤口愈合特性。这是由于纤维的已知仿生效应和含有缬沙坦的水凝胶促进的再上皮化增加的结果。本研究探索了使用仿生聚乳酸基电纺纤维和 HA-缬沙坦水凝胶支架结合局部血管紧张素受体阻滞剂成功加速伤口愈合。装载有透明质酸-缬沙坦水凝胶的新型 PLA 基电纺纤维是稳定的,并具有经证实的糖尿病伤口愈合特性。这是由于纤维的已知仿生效应和含有缬沙坦的水凝胶促进的再上皮化增加的结果。本研究探索了使用仿生聚乳酸基电纺纤维和 HA-缬沙坦水凝胶支架结合局部血管紧张素受体阻滞剂成功加速伤口愈合。装载有透明质酸-缬沙坦水凝胶的新型 PLA 基电纺纤维是稳定的,并具有经证实的糖尿病伤口愈合特性。这是由于纤维的已知仿生效应和含有缬沙坦的水凝胶促进的再上皮化增加的结果。
更新日期:2020-08-10
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