Abstract

Chronic alcohol or coffee/caffeine consumption has been associated with negative effects on male reproductive system. The present study evaluated the possible interaction (combination treatment) of coffee/caffeine and alcohol and its resultant consequence on sperm parameters in rats. Ten groups of adult Sprague Dawley rats (n = 5 per group) were orally gavaged with different quantities of coffee (≈5, 10, and 20 mg/kg caffeine); 30% v/v ethanol (0.5, 1, and 2 g/kg); coffee + ethanol or vehicle (distilled water) daily for 60 days. Sperm parameters, serum sex hormone levels, together with antioxidants and lipid peroxidation status of testis homogenate were evaluated. Single and combined administration of coffee/caffeine and ethanol caused a reduction in sperm count dose-dependently (p = 0.0002), but this effect was highest in the combined treatment. Sperm viability, motility, and morphology were unaffected by coffee or ethanol administration, but the combination produced detrimental effects on them. While changes in follicle stimulating hormone were not significant, testosterone and luteinizing hormone levels were decreased significantly and dose-dependently in all experimental groups (p < 0.05–0.0001). This effect was most pronounced in the groups that received the combination. Testicular superoxide dismutase and catalase activities, and reduced glutathione and malondiadehye (MDA) levels were not affected by coffee/caffeine, but ethanol decreased (p < 0.05) the antioxidants and elevated MDA levels mostly at 2 g/kg. The combination reduced (p < 0.0001) the antioxidants and increased MDA levels in testis homogenates at all doses. Combination of coffee/caffeine and ethanol potentiates their detrimental effects on sperm quality and reproductive hormones via reduction in testicular antioxidants activities and elevation of lipid peroxidation in rats.

摘要

长期饮酒或咖啡/咖啡因消费与男性生殖系统的负面影响有关。本研究评估了咖啡/咖啡因和酒精可能的相互作用（联合治疗）及其对大鼠精子参数的影响。10 组成年 Sprague Dawley 大鼠（每组n  = 5）用不同量的咖啡（≈5、10 和 20 mg/kg 咖啡因）进行口服灌胃；30% v/v 乙醇（0.5、1 和 2 g/kg）；每天咖啡 + 乙醇或载体（蒸馏水），持续 60 天。评估了精子参数、血清性激素水平以及睾丸匀浆的抗氧化剂和脂质过氧化状态。咖啡/咖啡因和乙醇的单一和联合给药导致精子数量减少，呈剂量依赖性（p = 0.0002），但这种效果在联合治疗中最高。精子活力、运动性和形态不受咖啡或乙醇给药的影响，但这种组合对它们产生了不利影响。虽然促卵泡激素的变化不显着，但所有实验组的睾酮和黄体生成素水平显着下降且呈剂量依赖性（p <  0.05-0.0001）。这种效果在接受组合的组中最为明显。睾丸超氧化物歧化酶和过氧化氢酶活性以及降低的谷胱甘肽和丙二醛（MDA）水平不受咖啡/咖啡因的影响，但乙醇降低（p <  0.05）抗氧化剂和升高MDA水平，主要在2 g / kg。组合减少 ( p < 0.0001) 在所有剂量的睾丸匀浆中的抗氧化剂和增加的 MDA 水平。咖啡/咖啡因和乙醇的组合通过降低大鼠睾丸抗氧化活性和提高脂质过氧化作用，增强了它们对精子质量和生殖激素的不利影响。