The total proteomes of Anisakis simplex s.s., A. pegreffii and their hybrid genotype have been compared by quantitative proteomics (iTRAQ approach), which considers the level of expressed proteins. Comparison was made by means of two independent experiments considering four biological replicates of A. simplex and two each for A. pegreffii and hybrid between both species. A total of 1811 and 1976 proteins have been respectively identified in the experiments using public databases. One hundred ninety-six proteins were found significantly differentially expressed, and their relationships with the nematodes’ biological replicates were estimated by a multidimensional statistical approach. Results of pairwise Log2 ratio comparisons among them were statistically treated and supported in order to convert them into discrete character states. Principal component analysis (PCA) confirms the validity of the method. This comparison selected thirty seven proteins as discriminant taxonomic biomarkers among A. simplex, A. pegreffii and their hybrid genotype; 19 of these biomarkers, encoded by ten loci, are specific allergens of Anisakis (Ani s7, Ani s8, Ani s12, and Ani s14) and other (Ancylostoma secreted) is a common nematodes venom allergen. The rest of the markers comprise four unknown or non-characterized proteins; five different proteins (leucine) related to innate immunity, four proteolytic proteins (metalloendopeptidases), a lipase, a mitochondrial translocase protein, a neurotransmitter, a thyroxine transporter, and a structural collagen protein. The proposed methodology (proteomics and statistical) solidly characterize a set of proteins that are susceptible to take advantage of the new targeted proteomics.

中文翻译：

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单纯异尖线虫 Sensu Stricto、A. pegreffii 及其杂交基因型总表达蛋白质组的定量蛋白质组学比较

通过定量蛋白质组学（iTRAQ 方法）对单纯异尖线虫、A. pegreffii 及其混合基因型的总蛋白质组进行了比较，该方法考虑了表达蛋白质的水平。通过两个独立实验进行了比较，考虑了 A. simplex 的四个生物复制品和 A. pegreffii 的两个生物复制品以及两个物种之间的杂交种。使用公共数据库在实验中分别鉴定了总共 1811 个和 1976 个蛋白质。发现一百九十六种蛋白质显着差异表达，并通过多维统计方法估计了它们与线虫生物重复的关系。对它们之间的成对Log2比率比较结果进行统计处理和支持，以便将它们转换成离散的字符状态。主成分分析（PCA）证实了该方法的有效性。该比较选择了 37 个蛋白质作为 A. simplex、A. pegreffii 及其混合基因型之间的区分分类生物标志物；其中 19 个生物标志物由 10 个位点编码，是异尖线虫的特异性过敏原（Ani s7、Ani s8、Ani s12 和 Ani s14），其他（钩虫分泌的）是常见的线虫毒液过敏原。其余标记包括四种未知或未表征的蛋白质；与先天免疫相关的五种不同蛋白质（亮氨酸）、四种蛋白水解蛋白（金属内肽酶）、脂肪酶、线粒体转位酶蛋白、神经递质、甲状腺素转运蛋白和结构胶原蛋白。所提出的方法（蛋白质组学和统计学）可靠地表征了一组易于利用新的目标蛋白质组学的蛋白质。