The spread of African swine fever virus (ASFV) leads to high mortality in domestic pigs, and has caused huge losses in the global pork industry. Therefore, the rapid detection of ASFV is essential for the prevention and control of the epidemic. Herein, a denaturation bubble‐mediated strand exchange amplification (SEA) with colorimetric detection strategy for the rapid detection of ASFV was established and the results were visible with the naked eyes. The entire detection process based on isothermal amplification was performed on a portable metal bath only. And the whole process including rapid extraction and visual detection was finished within 50 min. The limit of detection of this method reached to 10⁴ copies/μl of plasmid containing p72 gene. And the ASFV was successfully detected in eight different tissue and seven types of pig feed which revealed a strong anti‐interference ability in the presence of complex matrix. Through comparing 94 field positive and negative ASFV samples, excellent performance of SEA assay with 92.86% sensitivity and 100% specificity relative to PCR was observed. The developed method could be used for on‐site detection of ASFV with simplicity, rapidity, and visuality, especially suitable for pork industry food safety and ASFV control in resource‐limited areas.

中文翻译：

---

一种在生猪组织中检测非洲猪瘟病毒的视觉现场方法

非洲猪瘟病毒（ASFV）的传播导致家猪的高死亡率，并给全球猪肉业造成巨大损失。因此，快速检测ASFV对于预防和控制该流行病至关重要。在本文中，建立了带有比色检测策略的变性气泡介导的链交换扩增（SEA），用于快速检测ASFV，并且肉眼可见结果。基于等温扩增的整个检测过程仅在便携式金属浴上进行。并在50分钟内完成了包括快速提取和视觉检测在内的整个过程。此方法的检测极限达到10 ⁴含有p72基因的质粒的拷贝数/μl。并且在八种不同的组织和七种类型的猪饲料中成功检测出ASFV，这表明在存在复杂基质的情况下具有很强的抗干扰能力。通过比较94个田间阳性和阴性ASFV样品，观察到SEA分析的出色性能，相对于PCR灵敏度为92.86％，特异性为100％。所开发的方法可用于简便，快速和可视化的ASFV现场检测，尤其适用于猪肉行业食品安全和资源受限地区的ASFV控制。