Na⁺–K⁺–Cl⁻ cotransporter 2 (NKCC2; SLC12A1) is an integral membrane protein that comes as three splice variants and mediates the cotranslocation of Na⁺, K⁺, and Cl⁻ ions through the apical membrane of the thick ascending loop of Henle (TALH). In doing so, and through the involvement of other ion transport systems, it allows this nephron segment to reclaim a large fraction of the ultrafiltered Na⁺, Cl⁻, Ca²⁺, Mg²⁺, and HCO₃⁻ loads. The functional relevance of NKCC2 in human is illustrated by the many abnormalities that result from the inactivation of this transport system through the use of loop diuretics or in the setting of inherited disorders. The following presentation aims at discussing the physiological roles and molecular characteristics of Na⁺–K⁺–Cl⁻ cotransport in the TALH and those of the individual NKCC2 splice variants more specifically. Many of the historical and recent data that have emerged from the experiments conducted will be outlined and their larger meaning will also be placed into perspective with the aid of various hypotheses.

中文翻译：

---

Na+ -K+ -Cl-协同转运蛋白的分子特征和生理作用2。

Na ⁺ –K ⁺ –Cl ^-协同转运蛋白 2 (NKCC2; SLC12A1) 是一种完整的膜蛋白，以三种剪接变体形式出现，介导 Na ⁺、K ⁺和 Cl ^-离子通过厚上升环的顶膜的共转运亨勒 (TALH)。在这样做时，并通过其他离子转运系统的参与，它允许该肾单位段回收大部分超滤的 Na ⁺、Cl ^-、Ca ²⁺、Mg ^{2 +}和 HCO ₃ ^-负载。NKCC2 在人类中的功能相关性可以通过使用袢利尿剂或遗传性疾病导致该转运系统失活导致的许多异常来说明。以下介绍旨在更具体地讨论TALH中 Na ⁺ -K ⁺ -Cl ^-共转运的生理作用和分子特征以及单个 NKCC2 剪接变体的生理作用和分子特征。将概述从所进行的实验中出现的许多历史和最近数据，并且还将借助各种假设将其更大的意义置于透视中。