Research on the initial phage–host interaction has been conducted on a limited repertoire of phages and their cognate receptors, such as phage λ and the Escherichia coli LamB (EcLamB) protein. Apart from phage λ, little is known about other phages that target EcLamB. Here, we developed a simple method for isolating novel environmental phages in a predictable way, i.e. isolating phages that target a particular receptor(s) of a bacterium, in this case, the EcLamB protein. A plasmid (pMUT13) encoding the EcLamB porin was transferred into three different enterobacterial genera. By enrichment with these engineered bacteria, a number of phages (ZZ phages) that targeted EcLamB were easily isolated from the environment. Interestingly, although EcLamB‐dependent in their recombinant heterologous hosts, these newly isolated ZZ phages also targeted OmpC as an alternative receptor when infecting E. coli. Moreover, the phage host range was readily extended within three different bacterial genera with heterologously expressed EcLamB. Unlike phage λ, which is a member of the Siphoviridae family, these newly isolated EcLamB‐dependent phages were more commonly members of the Myoviridae family, based on transmission electron microscopy and genomic sequences. Modifications of this convenient and efficient phage enrichment method could be useful for the discovery of novel phages.

中文翻译：

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噬菌体宿主通过工程化的富集偏差，利用异源表面受体表达来进化宿主。

最初的噬菌体与宿主相互作用的研究是在有限的噬菌体及其同源受体库中进行的，例如噬菌体λ和大肠杆菌LamB（Ec LamB）蛋白。除了噬菌体λ外，对靶向Ec LamB的其他噬菌体知之甚少。在这里，我们开发了一种以可预测的方式分离新型环境噬菌体的简单方法，即分离靶向细菌特定受体（在这种情况下为Ec LamB蛋白）的噬菌体。将编码Ec LamB孔蛋白的质粒（pMUT13）转移到三个不同的肠杆菌属中。通过富集这些工程菌，许多针对Ec的噬菌体（ZZ噬菌体）LamB很容易与环境隔离。有趣的是，尽管Ec LamB依赖于其重组异源宿主，但这些新分离的ZZ噬菌体在感染E时也以OmpC为替代受体。大肠杆菌。此外，噬菌体宿主范围很容易在具有异源表达的Ec LamB的三个不同细菌属中扩展。与噬菌体λ（其是Siphoviridae家族的成员）不同，这些新分离的Ec LamB依赖性噬菌体更常见于肌病毒科的成员。家族，基于透射电子显微镜和基因组序列。这种方便有效的噬菌体富集方法的修改可能对发现新型噬菌体有用。