A new DNA architecture addresses the question, how far energy migrates in DNA and forms cyclobutane pyrimidine dimers (CPDs) as photodamages causing skin cancer. The 3‐methoxyxanthone nucleoside allows site‐selective photoenergy injection into DNA. The designated CPD site lacks the phosphodiester bond and can be placed in defined distances. The CPD formation links two oligonucleotides together and allows probing by gel electrophoresis. We obtained a sigmoidal distance dependence with R₀ of 25±3 Å. Below R₀, short‐range energy migration occurs with high CPD yields and shallow distance dependence, characteristic for a coherent process. 5‐methyl‐C as epigenetic modification on the 3′‐side facilitates CPD formation. Above R₀, long‐range incoherent energy migration occurs over 30 A‐T pairs (105.4 Å). The evidence of long‐range CPD formation is fundamental for our understanding of DNA photodamaging. Open access funding enabled and organized by Projekt DEAL.

中文翻译：

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能量在 DNA 中迁移多远并造成损伤？DNA 远距离光损伤的证据。

一种新的 DNA 结构解决了这样的问题：当光损伤导致皮肤癌时，能量在 DNA 中迁移多远并形成环丁烷嘧啶二聚体 (CPD)。3-甲氧基呫吨酮核苷允许位点选择性光能注入 DNA。指定的 CPD 位点缺乏磷酸二酯键，可以按规定的距离放置。CPD 形成将两个寡核苷酸连接在一起，并允许通过凝胶电泳进行探测。我们获得了R ₀为 25±3 Å 的S 形距离依赖性。低于R ₀时，会发生短程能量迁移，并具有高 CPD 产率和浅距离依赖性，这是相干过程的特征。5-甲基-C 作为 3' 侧的表观遗传修饰促进 CPD 的形成。高于R ₀时，超过 30 个 A-T 对 (105.4 Å) 会发生长程非相干能量迁移。长程 CPD 形成的证据对于我们理解 DNA 光损伤至关重要。由 Projekt DEAL 启用和组织的开放获取资金。