Abstract Cassava bacterial blight caused by Xanthomonas phaseoli p.v manihotis (Xpm) is a common disease of cassava (Manihot esculenta). The molecular mechanisms underlying susceptible and resistant response and host defense mechanisms are currently unknown. In this study, we performed large-scale mRNA expression profiling using an RNAseq approach to identify Cassava responses in MBRA685 variety inoculated with Xpm618 (resistant response) and Xpm318 (susceptible response) strains. Bioinformatic analysis revealed significantly differentially expressed genes (DEGs) by Xpm681 in MBRA685 with higher fold change values. Unique prevalent GO categories for Xpm681 response included “protein kinase activity” and “phosphorylation”; “amino acid biosynthetic process” for Xpm318 response and “regulation of transcription” and “transcription factor activity” in both responses. There were particular differences between the cultivars in the intensity of expression of genes with putative roles in transcriptional activity, receptor and signaling processes, secondary metabolism and photosynthesis. The differences between two interactions can be explained quantitatively, the resistant response was weaker than the susceptible response. Quantitative real-time PCR experiments confirmed the reliability of our RNAseq data.

中文翻译：

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对黄单胞菌的抗性和易感性 pv。木薯中的 Manihotis：转录组比较（或同一枚硬币的两侧）

摘要 由Xanthomonas phaseoli pv manihotis (Xpm) 引起的木薯白叶枯病是木薯(Manihot esculenta) 的常见病害。目前尚不清楚易感和抗性反应以及宿主防御机制的分子机制。在本研究中，我们使用 RNAseq 方法进行了大规模 mRNA 表达谱分析，以鉴定接种 Xpm618（抗性反应）和 Xpm318（易感反应）菌株的 MBRA685 品种中的木薯反应。生物信息学分析揭示了 Xpm681 在 MBRA685 中显着差异表达的基因 (DEG)，具有更高的倍数变化值。Xpm681 反应的独特流行 GO 类别包括“蛋白激酶活性”和“磷酸化”；Xpm318 反应的“氨基酸生物合成过程”和两种反应中的“转录调节”和“转录因子活性”。不同品种之间在转录活性、受体和信号传导过程、次级代谢和光合作用中具有推定作用的基因表达强度存在特殊差异。两种相互作用之间的差异可以定量解释，抗性反应弱于敏感反应。定量实时 PCR 实验证实了我们 RNAseq 数据的可靠性。两种相互作用之间的差异可以定量解释，抗性反应弱于敏感反应。定量实时 PCR 实验证实了我们 RNAseq 数据的可靠性。两种相互作用之间的差异可以定量解释，抗性反应弱于敏感反应。定量实时 PCR 实验证实了我们 RNAseq 数据的可靠性。