Hydrazinocurcumin is a semi-synthetic analogue of curcumin with superior anticancer and anti-angiogenic activities. In the present work a simple and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method for quantitative evaluation of hydrazinocurcumin in plasma and various organs of rats including liver, kidneys, brain, heart, lungs and spleen was developed. Hydrazinocurcumin was separated using octadecylsilane (Inertsil-ODS-3V) column in an isocratic mode using mobile phase consisting of methanol-acetonitrile- water (36:27:37 v/v) with flow rate of 1.0 ml/min. Ultra violet (UV) detection of hydrazinocurcumin and internal standard was carried out in dual-wavelength mode at 332 nm and 380 nm, respectively. The linearity of hydrazinocurcumin was found in the range 0.05–5 µg/ml with a correlation coefficient of r² > 0.999. The developed bioanalytical method shown higher inter-day accuracy (98.04–105.94%) and precision (0.89–10.24). The average recoveries of hydrazinocurcumin from rat plasma and various organs were in the range of 96–101.75% and 92.25–99.0%, respectively. The bioanalytical samples shows good stability of hydrazinocurcumin at different storage and handling conditions. In conclusion, this validated HPLC-UV method could be applied effectively for evaluation of hydazinocurcumin for the pharmacokinetic and organ distribution studies.

羟基姜黄素是姜黄素的半合成类似物，具有优异的抗癌和抗血管生成活性。在本工作中，开发了一种简单灵敏的反相高效液相色谱（RP-HPLC）方法，用于定量评估血浆和大鼠各种器官（包括肝，肾，脑，心脏，肺和脾脏）中的肼基姜黄素。使用十八烷基硅烷（Inertsil-ODS-3V）色谱柱以等度模式使用由甲醇-乙腈-水（36:27:37 v / v）组成的流动相以1.0 ml / min的流速分离肼基姜黄素。肼基姜黄素和内标的紫外（UV）检测分别在332 nm和380 nm的双波长模式下进行。肼基姜黄素的线性范围为0.05–5 µg / ml，相关系数为r²  > 0.999。先进的生物分析方法显示出更高的日间准确性（98.04–105.94％）和精度（0.89–10.24）。从大鼠血浆和各个器官中得到的肼基姜黄素的平均回收率分别为96–101.75％和92.25–99.0％。该生物分析样品在不同的储存和处理条件下显示出肼基姜黄素的良好稳定性。总之，这种经过验证的HPLC-UV方法可有效地用于评估肼基姜黄素的药代动力学和器官分布研究。