Molecularly imprinted polymers (MIPs) based on polydatin were prepared by precipitation polymerization method. Synthesis process of MIPs was optimized by discussion of functional monomers, porogens and the molar ratio of template- functional monomer-cross linker. Then, MIPs were prepared with polydatin as the template, 4-vinyl pyridine as the functional monomer, ethylene glycol dimethyl acrylate as the cross linker, acetonitrile as the porogen and the molar ratio of template-monomer-cross linker at 1:10:20. Scanning electron microscopy and Fourier transform infrared spectrometer were used to inspect macroscale and chemical bond of MIPs. Adsorption capability and selectivity of MIPs to polydatin were investigated by carrying out the static, dynamic and selective experiments. The results showed MIPs performed high adsorption ability and selectivity to polydatin, indicating MIPs could be used to separate and enrich polydatin from the complex systems. Finally, MIPs were applied as the adsorbent for isolation and purification of polydatin from the extract of Polygoni Cuspidati Rhizoma et Radix, rats' plasma and urine samples. MIPs were successfully used to separate polydatin from the Polygoni Cuspidati Rhizoma et Radix and recovery ranged from 89.2% to 91.6%. The maximum concentration of polydatin in rats' plasma and urine samples was 2.84 ± 0.0748 µg mL⁻¹ and 2.64 ± 0.485 µg mL⁻¹, respectively. Moreover, to compare with the MIPs method, organic solvent methods were used to analyze the polydatin in rats' plasma and urine samples. The results illustrated MIPs method was effective and selective for enrichment of polydatin from the medicinal plants and biological samples.

采用沉淀聚合法制备了基于多肽的分子印迹聚合物。通过讨论功能单体，成孔剂和模板功能单体交联剂的摩尔比，优化了MIP的合成工艺。然后，以多聚腺苷为模板，以4-乙烯基吡啶为功能单体，以乙二醇二甲基丙烯酸甲酯为交联剂，以乙腈为致孔剂，模板-单体-交联剂的摩尔比为1:10:20制备MIPs。 。扫描电子显微镜和傅立叶变换红外光谱仪用于检查MIPs的宏观和化学键。通过进行静态，动态和选择性实验，研究了MIP对多肽的吸附能力和选择性。结果表明，MIPs具有较高的吸附能力和对多肽的选择性，表明MIPs可用于从复杂系统中分离和富集多肽。最后，将MIPs用作吸附剂，从山茱of提取物中分离纯化白藜芦醇苷。虎杖川芎等沙参，大鼠血浆和尿液样品。的MIP被成功用于分离苷从虎杖川芎等沙参和回收率为89.2％至91.6％。大鼠血浆和尿液样品中的多甙的最大浓度分别为2.84±0.0748 µg mL ^-1和2.64±0.485 µg mL ^-1。此外，为了与MIPs方法进行比较，使用有机溶剂方法分析了大鼠血浆和尿液样品中的多甙。结果表明，MIPs方法对于从药用植物和生物样品中富集丰富的白藜芦醇苷是有效和选择性的。