In an attempt to mine cyanobacterial genes of potential biotechnological applications, the hypothetical protein All4894 of Anabaena PCC7120, following cloning, heterologous expression, purification and biochemical analysis, revealed the presence of a thermostable β-glucosidase. The glycosylated protein showed apparent molecular mass of ~18.0 kDa, while the purified enzyme depicted activity over wide pH (2.0–9.0) range and 85% stability up to 100 °C. All4894 encoding fascilin-1 domain showed higher specific activity to natural substrate cellobiose (Km = 0.75 mM and Vmax = 0.416 mMmin⁻¹ mg⁻¹) as compared to p-nitrophenyl-β-D-glucopyranoside. Quantitative reverse transcription polymerase chain reaction analysis (qRT-PCR) of all4894 revealed 4.0 to 16.0 fold elevated transcript under salinity, heat, arsenic, cadmium, UV-B and butachlor. The ectopic expression of All4894 in E. coli BL21 (DE3) reaffirmed its stress management capability. In view of the multiple functional attributes i.e. cell adhesion and abiotic stress tolerance All4894 may be regarded as “moonlighting protein” having a novel biomolecule for biotechnological applications including bioethanol production.

在尝试的潜在生物技术应用矿蓝藻的基因，的假定蛋白All4894鱼腥7120，如下克隆，异源表达，纯化和生物化学分析，揭示了热稳定的β葡糖苷酶的存在。糖基化蛋白的表观分子量约为18.0 kDa，而纯化的酶表现出在宽pH（2.0-9.0）范围内具有活性，在高达100°C的温度下具有85％的稳定性。与对-硝基苯基-β-D-吡喃葡萄糖苷相比，编码fascilin-1结构域的所有4894显示出对天然底物纤维二糖更高的比活性（Km = 0.75 mM和Vmax = 0.416 mMmin ^-1  mg ^-1）。定量逆转录聚合酶链反应分析（qRT-PCR）all4894在盐度，热，砷，镉，UV-B和丁草胺的作用下，其转录本升高了4.0到16.0倍。All4894在异位表达ë。大肠杆菌BL21（DE3）重申了其压力管理能力。考虑到多种功能属性，即细胞粘附和非生物胁迫耐受性，All4894可以被视为具有新型生物分子的“月光蛋白”，可用于包括生物乙醇生产在内的生物技术应用。