Modulation of the gut microbiota by probiotics, is emerging as a promising approach for the management of metabolic diseases but due to their species and strain specific response, isolation of new probiotic strains is gaining importance. The present study was designed to assess the effect of isolated and well characterised indigenous probiotics, Lactobacillus pentosus GSSK2, Lactobacillus fermentum PUM and Lactobacillus plantarum GS26A in high fat diet (HFD) induced metabolic syndrome. It was observed that though supplementation of all three probiotics for 12 weeks to Sprague Dawley rats fed with HFD, ameliorated the anthropometric parameters, but L. pentosus GSSK2 showed maximum reduction in weight gain while maximum decrease in abdominal circumference, Lee’s index, BMI and visceral fat deposition was observed in L. plantarum GS26A compared with HFD animals. Further, administration of L. plantarum GS26A to HFD animals led to significant increase in lactic acid bacteria count and lipid excretion in feces followed by L. pentosus GSSK2 and L. fermentum PUM compared with counter controls. Additionally, both L. pentosus GSSK2 and L. plantarum GS26A exhibited improved glucose tolerance, liver biomarkers, alleviated oxidative stress and restored the histoarchitechture of adipose tissue, colon and liver compared with HFD animals. The study highlights the prophylactic potential of isolated probiotics in experimental metabolic syndrome model and revealed that amongst all three probiotics, L. pentosus GSSK2 and L. plantarum GS26A were equally effective and more promising than L. fermentum PUM in improving metabolic dysfunctions and may be employed as functional foods but needs to be correlated clinically.

益生菌对肠道菌群的调节正在成为一种管理代谢疾病的有前途的方法，但是由于其种类和菌株特异性反应，分离新的益生菌菌株变得越来越重要。本研究旨在评估高脂饮食（HFD）诱导的代谢综合征中分离出的且特性良好的本地益生菌，戊糖乳杆菌GSSK2，发酵乳杆菌PUM和植物乳杆菌GS26A的作用。观察到，虽然向喂有HFD的Sprague Dawley大鼠补充了所有三种益生菌12周，但可以改善人体测量学参数，但戊糖乳杆菌与HFD动物相比，在植物乳杆菌GS26A中观察到GSSK2显示出最大的体重增加减少，而腹围，Lee's指数，BMI和内脏脂肪沉积最大减少。此外，与反对照相比，向HFD动物施用植物乳杆菌GS26A导致粪便中乳酸菌数量和脂质排泄显着增加，随后是戊糖乳杆菌GSSK2和发酵乳杆菌PUM。此外，戊糖乳杆菌GSSK2和植物乳杆菌与HFD动物相比，GS26A表现出改善的葡萄糖耐量，肝脏生物标志物，减轻的氧化应激并恢复了脂肪组织，结肠和肝脏的组织结构。该研究强调了分离的益生菌在实验性代谢综合征模型中的预防潜力，并揭示了在所有三种益生菌中，戊糖乳杆菌GSSK2和植物乳杆菌GS26A在改善代谢功能障碍方面均与发酵乳杆菌同等有效，并且前景广阔。作为功​​能食品，但需要在临床上进行关联。