Natural biologically active substances have received continuous attention for the potentially beneficial health properties against chronic diseases. In this study, bacteriostatic active substance from Camellia oleifera meal, which is a major by-product of the Camellia oil processing industry, were extracted with continuous phase change extraction (CPCE) method and separated by HSCCC. Compared with traditional extraction methods, CPCE possessed higher extraction efficiency. Two main substances were separated and purified (above 90.0%). The structure of them were further identified by UV, LC–ESI–MS–MS, 1H-NMR, and 13C-NMR as flavonoids F2 kaempferol 3-O-[β-d-glucopyranosyl-(1 → 2)-α-l-rhamnopyranosyl-(1 → 6)]-β-d-glucopyranoside and J2 kaempferol 3-O-[β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranosyl-(1 → 6)]-β-d-glucopyranoside for the first time in C. Oleifera meal. The results of antibacterial activity measurement showed that both compounds have excellent antibacterial activity. And the antibacterial stability of F2 were finally confirmed: F2 showed broad spectrum antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella enteriditis, Bacillus thuringiensis, Aspergillus niger and Rhizopus nigricans. Besides, F2 exhibited relatively high stable property even at high temperature, acid and metal ion solutions. The findings of this work suggest the possibility of employing C. oleifera meal as an attractive source of health-promoting compounds, and at the same time facilitate its high-value reuse and reduction of environmental burden.

天然生物活性物质因其对慢性疾病的潜在有益健康特性而受到持续关注。本研究采用连续相变萃取 (CPCE) 法提取油茶粕中的抑菌活性物质，这是油茶加工工业的主要副产品，并通过 HSCCC 分离。与传统的提取方法相比，CPCE具有更高的提取效率。两种主要物质被分离纯化（90.0%以上）。它们的结构通过紫外、LC-ESI-MS-MS、1H-NMR 和 13C-NMR 进一步鉴定为黄酮类 F2 山奈酚 3- O -[β- d-吡喃葡萄糖基-(1 → 2)-α -l-吡喃鼠李糖基-(1→6)]-β- d-吡喃葡萄糖苷和J2山奈酚3 - O- [β- d-吡喃木糖基- (1→2) -α- l-吡喃鼠李糖基- (1→6)]-β- d-吡喃葡萄糖苷首次出现在油菜粉中。抗菌活性测定结果表明，两种化合物均具有优良的抗菌活性。最终证实了F2的抗菌稳定性：F2对大肠杆菌、金黄色葡萄球菌、肠炎沙门氏菌、苏云金芽孢杆菌、黑曲霉和黑根霉具有广谱抗菌活性。. 此外，即使在高温、酸性和金属离子溶液中，F2也表现出较高的稳定性。这项工作的结果表明，可以使用油橄榄粉作为一种有吸引力的健康促进化合物来源，同时促进其高价值再利用和减少环境负担。