Telomerase is a specialized enzyme that attributes to maintaining and lengthening of telomeric length as its primary function. This ribonucleoprotein complex requires two minimum subunits for its in vivo function: the TERT amino acid subunit and TER its ribonucleic subunit. Crystallographic studies have shown that stable interaction between subunits and their domains is essential during the active phase. Plants and humans have shown to be tissue specific and dependent on cell proliferation. In Arabidopsis thaliana (L.) Heynh, the expression of AtTERT was determined to be necessary for activity. The objective of this work is to perform the in silico characterization of TERT in Agave tequilana F.A.C. Weber and evaluate the expression of TERT and the specific tissue activity in 2-year-old plants. To this end, we use the transcriptome library of A. tequilana (Ávila de Dios unpublished data) in TPM and qTRAP. We observe in this work that the consensus sequence shows all the specific domains of the TERT subunit. Additionally, we carried out the in silico prediction of the structural model of AteqTERT. The model presents the characteristic TERT ring of the crystallized models in active state of telomerase, biological annotations were observed, such as heavy metals Mg2+, NUC and CH1, and finally we note that, there is no correlation between TERT expression in TPM and telomerase activity in 2-year meristematic tissue.

中文翻译：

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田间条件下龙舌兰龙舌兰中 2 年生完整植株的 AteqTERT 表达和特定组织活性

端粒酶是一种特殊的酶，其主要功能是维持和延长端粒长度。这种核糖核蛋白复合物的体内功能需要两个最小的亚基：TERT 氨基酸亚基和 TER 其核糖核亚基。晶体学研究表明，亚基与其结构域之间的稳定相互作用在活跃阶段是必不可少的。植物和人类已被证明具有组织特异性并依赖于细胞增殖。在拟南芥 (L.) Heynh 中，AtTERT 的表达被确定为活动所必需的。这项工作的目的是在龙舌兰龙舌兰 FAC Weber 中对 TERT 进行计算机表征，并评估 TERT 的表达和 2 岁植物中的特定组织活性。为此，我们使用 A 的转录组库。TPM 和 qTRAP 中的龙舌兰酒（Ávila de Dios 未公开数据）。我们在这项工作中观察到，共有序列显示了 TERT 亚基的所有特定域。此外，我们对 AteqTERT 的结构模型进行了计算机预测。该模型呈现端粒酶活性状态下结晶模型的特征TERT环，观察到生物注释，如重金属Mg2+、NUC和CH1，最后我们注意到，TPM中的TERT表达与端粒酶活性之间没有相关性在 2 年的分生组织中。