Effective coverage and protection is a priority in wound treatment. Collagen and chitosan have been widely used for wound dressings due to their excellent biological activity and biocompatibility. Silver nanoparticles (AgNPs) have a powerful antibacterial effect. In this study, a macromolecular and small-molecular collagen mixed solution, a macromolecular and small-molecular chitosan mixed solution were prepared, and a silver nanoparticle-loaded collagen-chitosan dressing (AgNP-CCD) has been proposed. First, the effects of a collagen-chitosan mixed solution on the proliferation of human umbilical vein endothelial cells and the secretion of cytokines were evaluated. Then, the characteristics and antibacterial effects of the AgNP-CCD were tested, and the effects on wound healing and the influence of wound cytokine expression were investigated via a deep second-degree burn wound model. The results showed that at the proper proportion and concentration, the collagen-chitosan mixed solution effectively promoted cell proliferation and regulated the levels of growth factors (vascular endothelial growth factor [VEGF], epidermal growth factor [EGF], platelet-derived growth factor [PDGF], transforming growth factor [TGF-β1], basic fibroblastic growth factor [bFGF]) and inflammatory factors (TNF-α, IL-1β, IL-6, IL-8). Moreover, AgNP solutions at lower concentrations exerted limited inhibitory effects on cell proliferation and had no effect on cytokine secretion. The AgNP-CCD demonstrated satisfactory morphological and physical properties as well as efficient antibacterial activities. An in vivo evaluation indicated that AgNP-CCD could accelerate the healing process of deep second-degree burn wounds and played an important role in the regulation of growth and inflammatory factors, including VEGF, EGFL-7, TGF-β1, bFGF, TNF-α and IL-1β. This AgNP-CCD exerted excellent biological effects on wound healing promotion and cytokine expression regulation.

中文翻译：

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新型负载银纳米颗粒的胶原壳聚糖敷料的表征和生物学评估。

有效的覆盖和保护是伤口治疗的重点。胶原蛋白和壳聚糖由于其优异的生物活性和生物相容性而被广泛用于伤口敷料。银纳米颗粒（AgNPs）具有强大的抗菌作用。在这项研究中，制备了大分子和小分子的胶原蛋白混合溶液，大分子和小分子的壳聚糖混合溶液，并提出了载银纳米颗粒的胶原壳聚糖敷料（AgNP-CCD）。首先，评估胶原-壳聚糖混合溶液对人脐静脉内皮细胞增殖和细胞因子分泌的影响。然后，测试了AgNP-CCD的特性和抗菌效果，通过深度二级烧伤创面模型研究了其对创面愈合的影响以及创面细胞因子表达的影响。结果表明，在适当的比例和浓度下，胶原蛋白-壳聚糖混合溶液可有效促进细胞增殖并调节生长因子（血管内皮生长因子[VEGF]，表皮生长因子[EGF]和血小板衍生生长因子[ PDGF]，转化生长因子[TGF-β1]，碱性成纤维细胞生长因子[bFGF]）和炎性因子（TNF-α，IL-1β，IL-6，IL-8）。此外，较低浓度的AgNP溶液对细胞增殖的抑制作用有限，对细胞因子的分泌没有影响。AgNP-CCD表现出令人满意的形态和物理特性以及有效的抗菌活性。一个 胶原-壳聚糖混合溶液有效促进细胞增殖并调节生长因子的水平（血管内皮生长因子[VEGF]，表皮生长因子[EGF]，血小板衍生生长因子[PDGF]，转化生长因子[TGF-β1] ，碱性成纤维细胞生长因子[bFGF]）和炎性因子（TNF-α，IL-1β，IL-6，IL-8）。此外，较低浓度的AgNP溶液对细胞增殖的抑制作用有限，对细胞因子的分泌没有影响。AgNP-CCD表现出令人满意的形态和物理特性以及有效的抗菌活性。一个 胶原-壳聚糖混合溶液有效促进细胞增殖并调节生长因子的水平（血管内皮生长因子[VEGF]，表皮生长因子[EGF]，血小板衍生生长因子[PDGF]，转化生长因子[TGF-β1] ，碱性成纤维细胞生长因子[bFGF]）和炎性因子（TNF-α，IL-1β，IL-6，IL-8）。此外，较低浓度的AgNP溶液对细胞增殖的抑制作用有限，对细胞因子的分泌没有影响。AgNP-CCD表现出令人满意的形态和物理特性以及有效的抗菌活性。一个 转化生长因子[TGF-β1]，碱性成纤维细胞生长因子[bFGF]和炎症因子（TNF-α，IL-1β，IL-6，IL-8）。此外，较低浓度的AgNP溶液对细胞增殖的抑制作用有限，对细胞因子的分泌没有影响。AgNP-CCD表现出令人满意的形态和物理特性以及有效的抗菌活性。一个 转化生长因子[TGF-β1]，碱性成纤维细胞生长因子[bFGF]和炎症因子（TNF-α，IL-1β，IL-6，IL-8）。此外，较低浓度的AgNP溶液对细胞增殖的抑制作用有限，对细胞因子的分泌没有影响。AgNP-CCD表现出令人满意的形态和物理特性以及有效的抗菌活性。一个体内评价表明，AgNP-CCD可以加速深Ⅱ度烧伤创面的愈合过程，在调节血管内皮生长因子，EGFL-7，TGF-β1，bFGF，TNF-α等生长因子和炎症因子中起重要作用。和IL-1β。该AgNP-CCD在促进伤口愈合和调节细胞因子表达方面发挥了出色的生物学作用。