Bacterial species belonging to the genus Pseudobutyrivibrio are important members of the rumen microbiome contributing to the degradation of complex plant polysaccharides. Pseudobutyrivibrio xylanivorans MA3014 was selected for genome sequencing to examine its ability to breakdown and utilize plant polysaccharides. The complete genome sequence of MA3014 is 3.58 Mb, consists of three replicons (a chromosome, chromid and plasmid), has an overall G+C content of 39.6% and encodes 3,265 putative protein-coding genes (CDS). Comparative pan-genomic analysis of all cultivated and currently available P. xylanivorans genomes has revealed a strong correlation of orthologous genes within this rumen bacterial species. MA3014 is metabolically versatile and capable of growing on a range of simple mono- or oligosaccharides derived from complex plant polysaccharides such as pectins, mannans, starch and hemicelluloses, with lactate, butyrate and formate as the principal fermentation end-products. The genes encoding these metabolic pathways have been identified and MA3014 is predicted to encode an extensive range of Carbohydrate-Active enZYmes (CAZymes) with 80 Glycoside Hydrolases (GHs), 28 Carbohydrate Esterases (CEs) and 51 Glycosyl Transferases (GTs), suggesting an important role in solubilization of plant matter in the rumen.

中文翻译：

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完整的降解多糖的瘤胃细菌基因组序列假单胞菌木糖MA3014揭示了不完整的糖酵解途径。

属于伪丁酸弧菌属的细菌物种是瘤胃微生物组的重要成员，有助于降解复杂的植物多糖。选择拟南丁假单胞菌MA3014进行基因组测序，以检测其分解和利用植物多糖的能力。MA3014的完整基因组序列为3.58 Mb，由三个复制子（染色体，染色体和质粒）组成，总G + C含量为39.6％，编码3265个推定的蛋白质编码基因（CDS）。对所有栽培的和目前可获得的木对苯二酚的比较全基因组分析基因组已揭示该瘤胃细菌物种中直系同源基因的强相关性。MA3014具有新陈代谢功能，能够在多种简单的单糖或寡糖上生长，这些单糖或寡糖以复杂的植物多糖（如果胶，甘露聚糖，淀粉和半纤维素）为主要原料，其中乳酸，丁酸和甲酸为主要发酵终产物。已经鉴定出编码这些代谢途径的基因，并且预测MA3014可以编码多种糖活性酶（CAZymes），其中含有80种糖苷水解酶（GH），28种糖基酯酶（CE）和51种糖基转移酶（GTs），这表明在瘤胃中植物物质的溶解中起重要作用。