One of the key mechanisms of alcoholic liver disease is oxidative stress. Both Curcumin and Baicalin exert antioxidant effects, but the mechanism of their combined effects of ethanol‐induced liver injury is still unclear. This study was conducted to evaluate the dual antioxidant activity of Curcumin combined with Baicalin against ethanol‐induced liver injury in rats. Rats were divided into five groups, a control, ethanol, ethanol + Curcumin (50 mg/kg), ethanol + Baicalin (50 mg/kg), and ethanol + Curcumin +Baicalin group with ten rats per group. The effects of ethanol on liver enzymes, oxidative stress indicators and the levels of Nrf2/HO‐1 pathway related proteins and mRNA were observed along with liver histopathology in rats. Our results found that the serum ALT and AKP levels were increased in ethanol‐treated rats, which also showed a rising trend of 8‐OHdG and LPO levels while hydroxyl radical scavenging ability, T‐AOC, and the activities of SOD and GSH‐Px were decreased in liver. The mRNA levels of Nrf2 and HO‐1, the ratio of p‐Nrf2/Nrf2, the protein level of HO‐1 were decreased while NQO1 mRNA level, Nrf2, p‐Nrf2, and NQO1 protein levels were increased in ethanol‐treated rats. Combination treatment of Curcumin and Baicalin significantly reversed the ethanol‐induced liver oxidative damage and further activate the Nrf2/HO‐1 pathway, which was more effective than each drug alone. In conclusion, evidence has shown for the first time in this study that Curcumin combined with Baicalin ameliorated ethanol‐induced liver oxidative damage in rats and revealed liver‐protection.

中文翻译：

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姜黄素和黄ical苷可通过Nrf2 / HO-1途径减轻乙醇诱导的肝氧化损伤。

酒精性肝病的关键机制之一是氧化应激。姜黄素和黄ical苷均具有抗氧化作用，但是乙醇诱导的肝损伤的联合作用机制仍不清楚。这项研究旨在评估姜黄素联合黄ical苷对大鼠乙醇诱导的肝损伤的双重抗氧化活性。将大鼠分为五个组，对照组，乙醇，乙醇+姜黄素（50mg / kg），乙醇+黄ical苷（50mg / kg）和乙醇+姜黄素+黄ical苷组，每组十只大鼠。观察到乙醇对大鼠肝脏酶，氧化应激指标以及Nrf2 / HO-1通路相关蛋白和mRNA水平的影响，以及肝脏组织病理学。我们的结果发现，乙醇治疗的大鼠血清ALT和AKP水平升高，肝脏中8-OHdG和LPO含量也呈上升趋势，而羟自由基清除能力，T-AOC以及SOD和GSH-Px活性却下降。的mRNA水平在乙醇处理的大鼠中，Nrf2和HO-1，p-Nrf2 / Nrf2的比率，HO-1的蛋白质水平降低，而NQO1 mRNA水平，Nrf2，p-Nrf2和NQO1蛋白质水平升高。姜黄素和黄ical苷的联合治疗显着逆转了乙醇诱导的肝氧化损伤，并进一步激活了Nrf2 / HO-1途径，这比单独使用每种药物更有效。总之，本研究首次显示姜黄素联合黄with苷可改善乙醇诱导的大鼠肝脏氧化损伤并显示对肝脏的保护作用。