Aspergillus terreus can produce different holocellulose‐degrading enzymes when grown in sugarcane bagasse, with predominant pectinase activity. Thus, pectinase was selected for purification and immobilization studies. Ion exchange and molecular exclusion chromatography studies were performed, after which it was possible to semipurify the enzyme with a yield of 80%. The crude extract pectinase (PECEB) and the partially purified enzyme (PEC2) were immobilized on monoamino‐N‐aminoethyl (MANAE)‐agarose with pectinase activity yields of 66% and 98%, respectively. After immobilization in MANAE‐agarose, the pectinase showed higher activity at acidic pH (pH 4.0) when compared to the nonimmobilized enzyme. It was also found that after the immobilization process, there was a threefold improvement in the enzyme's thermostability. Also, it was possible to reuse the immobilized enzyme for up to five cycles of hydrolysis with effective production of reducing sugars (0.196 mg/g of substrate). The industrial application test revealed a significant decrease in the viscosity of guava juice when the immobilized enzyme was used. PECEB, immobilized on MANAE‐agarose, was the enzyme sample that generated the highest pulp viscosity reduction (approximately 47%). Although additional studies are needed for practical industrial application, the results obtained herein reveal the potential of application of immobilized pectinase in the industry.

中文翻译：

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土曲霉产生的果胶酶的固定化研究

在甘蔗渣中生长时，曲霉曲霉会产生不同的全纤维素降解酶，并具有主要的果胶酶活性。因此，选择了果胶酶用于纯化和固定化研究。进行了离子交换和分子排阻色谱研究，此后有可能以80％的产率对酶进行半纯化。粗提果胶酶（PECEB）和部分纯化的酶（PEC2）固定在单氨基-N上氨基胶（MANAE）-琼脂糖的果胶酶活性分别为66％和98％。与未固定化的酶相比，固定在MANAE-琼脂糖中后，果胶酶在酸性pH（pH 4.0）下显示出更高的活性。还发现在固定过程之后，酶的热稳定性提高了三倍。同样，可以将固定化的酶重复使用多达五个水解周期，从而有效产生还原糖（0.196 mg / g底物）。工业应用测试表明，当使用固定化酶时，番石榴汁的粘度显着降低。固定在MANAE-琼脂糖上的PECEB是产生最大果肉粘度降低（约47％）的酶样品。