The bioreactor flow environment has a significant impact on process performance, especially in stem cell cultures. The work of Correia et al found intermittent agitation modes to improve induced pluripotent stem cell (iPSC)‐cardiomyocyte differentiation yields; however, to date, the impact within the flow has not been fully characterized. This work aims to characterize the flow dynamics occurring within a commercially available DASGIP bioreactor, equipped with a two‐blade paddle impeller, operating under different agitation modes and for two bottom geometries. The paddle impeller configuration generated an axial flow profile due to a large impeller D/T and blade confinement with the bioreactor wall. The application of intermittent agitation was shown to induce two transient spikes in flow velocity and shear stress, the amplification of which increased with dwell duration. Marginally increasing the dwell duration was shown previously to increase differentiation yields, therefore it can be stipulated that introduction of these spikes was favorable toward cardiogenic differentiation.

中文翻译：

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DASGIP生物反应器中的流动，悬浮和混合动力学：第1部分

生物反应器的流动环境对工艺性能有重大影响，尤其是在干细胞培养中。Correia等人的工作发现间歇性搅拌模式可提高诱导性多能干细胞（iPSC）-心肌细胞的分化能力；但是，到目前为止，流程中的影响尚未完全确定。这项工作旨在表征在装有双叶片桨叶轮的市售DASGIP生物反应器中发生的流动动力学，该叶轮在不同的搅拌模式下运行并适用于两个底部几何形状。桨叶轮的配置由于大的叶轮D / T而产生了轴向流分布和叶片限制在生物反应器壁上。结果表明，间歇性搅拌会引起流速和剪切应力两个瞬时峰值，其放大率随停留时间的增加而增加。先前已证明稍微延长停留时间会增加分化的产率，因此可以规定引入这些尖峰有利于心源性分化。