Cyanobacteria are attracting the attention worldwide as a reliable and sustainable feedstock for the production of biofuels, food colorants and biochemically active compounds. Anabaena cylindrica (A. cylindrica) is a cyanobacterium with a significant widespread occurrence in Portuguese freshwaters, with C-phycocyanin and chlorophylls, compounds with a high market value, being abundantly produced by the species. This work presents the development of a sequential downstream process to obtain C-phycocyanin and chlorophyll from A. cylindrica. The C-phycocyanin extraction is carried using Na-phosphate (20 mM, pH 7) buffer, while aqueous solutions of surface-active compounds (250 mM), as well as ethanol, were screened for the extraction of the chlorophylls. After selecting the best solvents to recover both pigments from A. cylindrica, the operational conditions (solid-liquid ratio, time of extraction and temperature) were optimized, allowing the recovery of around 90% and 55% of the total content of C-phycocyanin and chlorophylls, respectively. Finally, dextran/copolymer-based ABS were applied to promote the purification of C-phycocyanin from the residual chlorophylls and other contaminant proteins, leading to an increase in the C-phycocyanin purity of 4-fold, without compromising its photostability.

蓝细菌作为生产生物燃料，食用色素和生物化学活性化合物的可靠和可持续的原料正吸引着全世界的目光。鱼腥藻（A. cylindrica）是一种蓝藻细菌，在葡萄牙的淡水中有广泛的分布，C-藻蓝蛋白和叶绿素是一种具有很高市场价值的化合物，被该物种大量生产。这项工作提出了一个顺序的下游过程的发展，从A. cylindrica获得C-藻蓝蛋白和叶绿素。使用磷酸钠（20 mM，pH 7）缓冲液进行C-藻蓝蛋白提取，同时筛选表面活性化合物（250 mM）的水溶液以及乙醇，以提取叶绿素。在选择最佳溶剂以从圆环曲霉中回收两种色素后，优化了操作条件（固液比，提取时间和温度），回收率约为C-藻蓝蛋白总含量的90％和55％。和叶绿素。最后，使用基于葡聚糖/共聚物的ABS促进从残留叶绿素和其他污染物蛋白中纯化C-藻蓝蛋白，从而使C-藻蓝蛋白的纯度提高4倍，而不会损害其光稳定性。