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SARS-CoV-2-specific ELISA development.
Journal of Immunological Methods ( IF 2.2 ) Pub Date : 2020-08-08 , DOI: 10.1016/j.jim.2020.112832
Vicky Roy 1 , Stephanie Fischinger 1 , Caroline Atyeo 1 , Matthew Slein 1 , Carolin Loos 2 , Alejandro Balazs 1 , Corinne Luedemann 1 , Michael Gerino Astudillo 3 , Diane Yang 3 , Duane R Wesemann 4 , Richelle Charles 5 , A John Lafrate 3 , Jared Feldman 1 , Blake Hauser 1 , Tim Caradonna 1 , Tyler E Miller 3 , Mandakolathur R Murali 3 , Lindsey Baden 4 , Eric Nilles 4 , Edward Ryan 5 , Douglas Lauffenburger 6 , Wilfredo Garcia Beltran 7 , Galit Alter 1
Affiliation  

Critical to managing the spread of COVID-19 is the ability to diagnose infection and define the acquired immune response across the population. While genomic tests for the novel Several Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) detect the presence of viral RNA for a limited time frame, when the virus is shed in the upper respiratory tract, tests able to define exposure and infection beyond this short window of detectable viral replication are urgently needed. Following infection, antibodies are generated within days, providing a durable read-out and archive of exposure and infection. Several antibody tests have emerged to diagnose SARS-CoV-2. Here we report on a qualified quantitative ELISA assay that displays all the necessary characteristics for high-throughput sample analysis. Collectively, this test offers a quantitative opportunity to define both exposure and levels of immunity to SARS-CoV-2.



中文翻译:

SARS-CoV-2 特异性 ELISA 开发。

控制 COVID-19 传播的关键是能够诊断感染并定义人群中的获得性免疫反应。虽然新型几种急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 的基因组测试在有限的时间内检测到病毒 RNA 的存在,但当病毒在上呼吸道传播时,测试能够定义超出范围的暴露和感染。迫切需要这种可检测的病毒复制的短窗口。感染后,抗体会在几天内产生,从而提供暴露和感染的持久读数和存档。已经出现了几种用于诊断 SARS-CoV-2 的抗体测试。在这里,我们报告了合格的定量 ELISA 测定,该测定显示了高通量样品分析的所有必要特征。总的来说,该测试提供了一个定量的机会来定义 SARS-CoV-2 的暴露程度和免疫水平。

更新日期:2020-09-08
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