Abstract Bioremediation is an attractive strategy of utilizing bacteria to remove crude oil contaminants. In this study, two salt-tolerant crude oil-degrading and biosurfactant-producing bacteria, Dietzia sp. CN-3 and Acinetobacter sp. HC8–3S, were functionally combined to construct a bacterial consortium. The consortium achieved 95.8% degradation efficiency of crude oil in 10 days and various n-alkanes, cycloalkanes, branched alkanes and aromatic hydrocarbons were all depleted more effectively than single strains. Functional optimization of the consortium degraded crude oil efficiently in a wide range of pH (4–10) and salinity (0–120 g L−1). Furthermore, two alkane hydroxylase genes, alkB in CN-3 and alkM in HC8–3S, were cloned and their expression were examined by real-time quantitative polymerase chain reaction, indicating that alkB was more prominent in long-chain alkanes (C20, C24 and C26) utilization and alkM played crucial roles in medium- and long-chain alkanes (C14, C16, C20, C24 and C26) degradation. In soil microcosms artificially contaminated with crude oil and bioaugmented with the consortium, 58.3% of total petroleum hydrocarbons were depleted after 60 days and the degradation rate (485.8 mg kg−1 d−1) was higher than those reported in previous studies. Consequently, the consortium is a promising candidate in crude oil bioremediation.

中文翻译：

---

通过由耐盐石油降解剂和生物表面活性剂生产商构成的细菌聚生体增强原油的生物降解

摘要 生物修复是一种利用细菌去除原油污染物的有吸引力的策略。在这项研究中，两种耐盐原油降解和生物表面活性剂产生细菌 Dietzia sp。CN-3 和不动杆菌属。HC8-3S，在功能上结合以构建细菌聚生体。该联合体在10天内实现了95.8%的原油降解效率，各种正烷烃、环烷烃、支链烷烃和芳烃的去除效率均高于单一菌株。财团的功能优化在很宽的 pH (4-10) 和盐度 (0-120 g L-1) 范围内有效降解原油。此外，克隆了两个烷烃羟化酶基因，CN-3 中的 alkB 和 HC8-3S 中的 alkM，并通过实时定量聚合酶链反应检测了它们的表达，表明 alkB 在长链烷烃（C20、C24 和 C26）利用中更为突出，alkM 在中链和长链烷烃（C14、C16、C20、C24 和 C26）降解中起关键作用。在被原油人工污染并用财团生物增强的土壤微观世界中，60 天后总石油烃的 58.3% 被耗尽，降解率（485.8 mg kg-1 d-1）高于先前研究报告的那些。因此，该财团是原油生物修复的有希望的候选者。60 天后，总石油烃的 3% 被耗尽，降解率 (485.8 mg kg-1 d-1) 高于先前研究报告的那些。因此，该财团是原油生物修复的有希望的候选者。60 天后，总石油烃的 3% 被耗尽，降解率 (485.8 mg kg-1 d-1) 高于先前研究报告的那些。因此，该财团是原油生物修复的有希望的候选者。