Interest in xylanase enzyme application has led to production of xylanase from recombinant Escherichia coli B24, which is an economic alternative towards higher productivity. Recombinant E. coli used in this study is a ubiquitous bacterium containing xylanase encoding gene from Bacillus halodurans. We investigated xylanase production by recombinant E. coli using classical medium optimization. Six fermentation media had been chosen from literature for xylanase production. Afterwards, the most suitable medium was further optimized by varying the key nutrients. The final optimized cultivation medium consisted of (g.L^-1): glucose, 2.5; NH₄Cl, 0.4; KH₂PO₄, 3.0; Na₂HPO₄, 6.0; MgSO₄.7H₂O, 1.0. After medium optimization, maximal volumetric xylanase production (600.25 U.mL^-1) increased by about 115.22% from the initial un-optimized medium (278.9 U.mL^-1).

中文翻译：

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重组大肠杆菌B24生产木聚糖酶的培养基优化

对木聚糖酶应用的兴趣已导致从重组大肠杆菌B24生产木聚糖酶，这是提高生产率的经济选择。本研究中使用的重组大肠杆菌是一种普遍存在的细菌，含有嗜盐芽孢杆菌的木聚糖酶编码基因。我们使用经典培养基优化方法研究了重组大肠杆菌生产的木聚糖酶。从文献中选择了六种发酵培养基用于木聚糖酶的生产。之后，通过改变关键营养成分进一步优化了最合适的培养基。最终优化的培养基由（gL ^-1）组成：葡萄糖2.5。NH ₄ Cl，0.4；KH ₂ PO₄，3.0; 的Na ₂ HPO ₄，6.0; MgSO ₄ .7H ₂ O，1.0。培养基优化后，最大的木聚糖酶总产量（600.25 U.mL ^-1）比未优化的初始培养基（278.9 U.mL ^-1）增加了约115.22％。