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Metabolic Phenotyping Study of Mouse Brains Following Acute or Chronic Exposures to Ethanol.
Journal of Proteome Research ( IF 4.4 ) Pub Date : 2020-08-07 , DOI: 10.1021/acs.jproteome.0c00440
Olga Deda 1, 2 , Christina Virgiliou 2, 3 , Emily G Armitage 4 , Amvrosios Orfanidis 1 , Ioannis Taitzoglou 5 , Ian D Wilson 6 , Neil Loftus 4 , Helen G Gika 1, 2
Affiliation  

The chronic and acute effect of ethanol administration on the metabolic phenotype of mouse brain was studied in a C57BL/6 mouse model of ethanol abuse using both untargeted and targeted ultra performance liquid chromatography-tandem mass spectrometry. Two experiments based on either chronic (8 week) exposure to ethanol of both male and female mice or acute exposure of male mice for 11 days, plus 2 oral gavage doses of 25% ethanol, were undertaken. Marked differences were found in amino acids, nucleotides, nucleosides, and related metabolites as well as a number of different lipids. Using untargeted metabolite profiling, acute ethanol exposure found significant decreases in several metabolites including nucleosides, fatty acids, glycerophosphocholine, and a number of phospholipids, while chronic exposure resulted in increases in several amino acids with notable decreases in adenosine, acetylcarnitine, and galactosylceramides. Similarly, targeted metabolite analysis, focusing on the hydrophilic fraction of the brain tissue extract, identified significant decreases in the metabolism of amino acids and derivatives, as well as purine degradation especially after chronic exposure to ethanol.

中文翻译:

小鼠急性或慢性暴露于乙醇后的代谢表型研究。

在C57BL / 6乙醇滥用小鼠模型中,使用非靶向和靶向超高效液相色谱-串联质谱法研究了乙醇给药对小鼠大脑代谢表型的慢性和急性作用。基于雄性和雌性小鼠长期(8周)暴露于乙醇或雄性小鼠急性暴露11天,再加上两次管饲剂量的25%乙醇,进行了两项实验。在氨基酸,核苷酸,核苷和相关代谢产物以及许多不同的脂质中发现明显差异。使用非靶向代谢物谱,急性乙醇暴露发现几种代谢物(包括核苷,脂肪酸,甘油磷酸胆碱和许多磷脂)显着降低,而长期暴露会导致几种氨基酸的增加,而腺苷,乙酰肉碱和半乳糖基神经酰胺则明显减少。同样,针对目标代谢物的分析,集中在大脑组织提取物的亲水部分上,发现氨基酸和衍生物的代谢显着下降,以及嘌呤降解,特别是在长期暴露于乙醇后。
更新日期:2020-10-02
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