The objective of present work was to isolate the mucilage as polysaccharide from the seeds of Cassia obtustifolia and to modify the isolated polysaccharide chemically by thiolation and carboxymethylation to develop novel excipient for drug delivery application. The attempts were made to enhance mucoadhesive potential of Cassia obtustifolia seed mucilage by thiolation and to reduce the disintegration time in prepared tablets by carboxymethylation. Thiolation of Cassia obtustifolia seed mucilage was achieved by esterification with thioglycolic acid and thiol group content was measured by using Ellman’s reagent, whereas carboxymethylation was archived by etherification of Cassia obtusifolia seed mucilage with monochloroacetic acid and the degree of carboxymethyl substitution was determined by titrimetric method. Thiolation and carboxymethylation of Cassia mucilage was confirmed by, Fourier transform infrared spectroscopy (FT-IR), ¹H NMR spectroscopy, differential scanning calorimeter (DSC thermogram) and by Zeta potential measurement. The findings demonstrated that there is significant improvement in bioadhesive and disintegration property via respective chemical modifications such as thiolation and carboxymethylation.

当前工作的目的是从决明子的种子中分离作为多糖的粘液，并通过硫醇化和羧甲基化对分离出的多糖进行化学修饰，以开发出新的赋形剂，以用于药物递送。尝试通过硫醇化来增强决明子种子粘液的粘膜粘附潜能，并通过羧甲基化减少制得的片剂中的崩解时间。通过用巯基乙酸酯化来实现对决明子种子粘液的硫醇化作用，并使用Ellman试剂测量巯基含量，而通过对决明子进行醚化来保存羧甲基化用滴定法测定一氯乙酸对种子的黏性和羧甲基取代度。决明胶的硫醇化和羧甲基化通过傅里叶变换红外光谱（FT-IR），¹ H NMR光谱，差示扫描量热仪（DSC温度谱图）和Zeta电位测定得到证实。这些发现表明，通过相应的化学修饰（例如硫醇化和羧甲基化），生物粘附性和崩解性有了显着改善。