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Physiological response and removal ability of freshwater diatom Nitzschia palea to two organophosphorus pesticides
Chemistry and Ecology ( IF 2.5 ) Pub Date : 2020-08-07
Yuhang Wang, Weijie Mu, Xiaoli Sun, Xinxin Lu, Yawen Fan, Yan Liu

Organophosphorus pesticides, one of the most extensively used organic chemicals to control several insect pests, making them threaten the aquatic creatures. Here, we analysed the response mechanism of Nitzschia palea to insecticides (acephate and trichlorfon) exposure, through physiological, biochemical, morphological changes and the removal ability. Results showed that the growth of N. palea was inhibited by trichlorfon, with the cell density was significantly decreased at 7 days of exposure. Although the diatom growth was hardly inhibited after acephate treatment, the diatom valve significantly abnormal morphology be found at 50 mg L−1 acephate treatment after 7 days. Meanwhile, these insecticides had no negative effect on the chlorophyll a content of N. palea. In addition, acephate and trichlorfon induced the activities of superoxide dismutase (SOD) and catalase (CAT) in the diatom, which play a defensive role against various environmental stresses. For further analysis revealed that the content of malondialdehyde (MDA) and extracellular polysaccharides (EPS) increased on exposure to both insecticides. N. palea also promoted 24.44–32.75% removal of acephate. The biochemical changes in N. palea on exposure to acephate and trichlorfon indicate that the diatom undergoes an adaptive change in response to the insecticides-induced oxidative stress.



中文翻译:

淡水硅藻(Nitzschia palea)对两种有机磷农药的生理响应和去除能力

有机磷农药是控制多种害虫的最广泛使用的有机化学物质之一,使其威胁水生生物。在这里,我们通过生理,生化,形态学变化和去除能力,分析了纳氏菌对杀虫剂(乙酰磷酸盐和敌百虫)暴露的响应机制。结果表明,敌百虫可抑制猪笼草的生长,暴露7天后细胞密度显着降低。尽管在乙酰乙酸盐处理后几乎没有抑制硅藻的生长,但是在7天后50 mg L -1乙酰磷酸盐处理后,发现硅藻阀的形态明显异常。同时,这些杀虫剂对叶绿素a没有负面影响。猪笼草的含量此外,乙酰甲胺磷和敌百虫还诱导了硅藻中的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性,对各种环境胁迫起着防御作用。进一步分析表明,丙二醛(MDA)和细胞外多糖(EPS)的含量在接触两种杀虫剂时均增加。北部猪笼草还促进了乙酸乙酯的24.44-32.75%去除。暴露于乙酰甲酸盐和敌百虫时,印度猪笼草的生化变化表明,硅藻响应于杀虫剂诱导的氧化应激而经历了适应性变化。

更新日期:2020-08-08
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