The discovery and deployment of new broad-spectrum resistance (R) genes from cultivated rice and its wild relatives is a strategy to broaden the genetic basis of modern rice cultivars to combat rice blast disease. Oryza glaberrima possessing many valuable traits for tolerance to biotic and abiotic stresses, is an elite gene pool for improvement of Asian cultivated rice. An introgression line IL106 derived from O. glaberrima (Acc. IRGC100137) confers complete resistance to Magnaporthe oryzae in blast nursery. Genetic analysis using 2185 BC₆F₂ progenies derived from a cross between IL106 and the recurrent parent Dianjingyou 1 showed that IL106 harbors a single dominant resistance gene against M. oryzae strain 09BSH-10-5A. This gene was preliminarily mapped on the long arm of chromosome 6 of rice in a region of ca. 0.9 cM delimited by two SSR markers (RM20650 and RM20701). In order to finely map this gene, 17,100 additional progenies were further analyzed. As a result, this gene was further narrowed down to a region flanked by two molecular markers STS69-15 and STS69-7, and co-segregated with 3 molecular markers, RM20676, STS69-21 and STS69-22 on the long arm of chromosome 6. Based on reference genome sequences, this R gene was mapped in silico in 76.1-Kb and 67.7-Kb physical intervals, and containing 4 and 3 NBS-LRR candidate genes in O. sativa cultivar Nipponbare and O. glaberrima cultivar CG14, respectively. Because no blast resistance gene was finely mapped in this physical interval before, this R gene was considered as not described yet and designated as Pi69(t), which is the first identified and finely mapped blast R gene from O. glaberrima, as far as we know. Evaluation of IL106 with 151 blast strains collected from 6 countries in Asia showed that 148 strains are avirulent on IL106, suggesting that Pi69(t) is a broad-spectrum blast R gene, and a promising resistant resource for improvement of Asian cultivated rice.

新广谱抗药性的发现和部署（[R）从栽培水稻及其野生近缘种获得的基因是一种拓宽现代水稻品种的遗传基础以抗击稻瘟病的策略。 稻具有对生物和非生物胁迫耐受性的许多宝贵特征，是改良亚洲栽培稻的优良基因库。渗入线IL106来自O. glaberrima （ACC。IRGC100137）赋予完全抵抗力 稻瘟病菌e在爆炸苗圃。使用从IL106和轮回亲本滇粳优1杂交得到的2185 BC ₆ F ₂子代进行的遗传分析表明，IL106带有单个抗性基因米曲霉菌株09BSH-10-5A。该基因被初步定位在水稻大约6号染色体的长臂上。0.9 cM由两个SSR标记（RM20650和RM20701）定界。为了精确定位该基因，进一步分析了17,100个其他后代。结果，该基因进一步缩小到两个分子标记STS69-15和STS69-7侧翼的区域，并与3个分子标记RM20676，STS69-21和STS69-22在染色体长臂上共同分离6.根据参考基因组序列，[R 该基因在76.1-Kb和67.7-Kb的物理区间进行了计算机定位，并分别包含4和3个NBS-LRR候选基因。 水稻 品种Nipponbare和 O. glaberrima品种CG14。由于之前没有在此物理间隔中精确定位抗稻瘟病基因，因此[R 该基因被认为尚未描述，并被指定为 Pi69（t），这是第一个被识别并精确定位的爆炸 [R 来自的基因 O. glaberrima， 据我们了解。用从亚洲6个国家/地区收集的151株blast菌株对IL106进行评估，结果显示148株在IL106上无毒。Pi69（t）是广谱爆炸 [R 基因，是改善亚洲栽培稻的有希望的抗性资源。