Chemical composition of biomass, especially carbohydrate content, is a critical indicator of a biomass source’s potential for biofuel applications. This study characterized physico-chemical properties of stalks from 16 representative pedigreed sorghum mutant lines. The objectives of this study were to evaluate the recovery of sucrose and its hydrolysis products, glucose and fructose, during dilute sulfuric acid pretreatment at conditions typically used for lignocellulosic biomass, and to determine the relationship between water-extractive contents and sugar recovery after pretreatment. Dilute acid-pretreated sorghum stalks had enzymatic saccharification of >82.4% glucose yield for all treated samples with more than 82.3% cellulose recovery and 85% hemicellulose removal. A single-step, one-pot process was recommended for sorghum mutant stalks with low water-extractive content (<35%, w/w) to reduce processing cost and minimize wastewater disposal since the majority of sugars will be recovered after dilute acid pretreatment with minimal degradation products. However, for sorghum mutant stalks with high water-extractive content (>35%, w/w), a pre-washing step is beneficial to recover the water-soluble sugars before subjecting to the pretreatment process in order to avoid sugar losses during the pretreatment stage. Thus, different processing technologies should be applied to lignocellulosic biomass with various water-extractive contents and water-soluble sugar concentrations.

中文翻译：

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高粱突变体秸秆的水溶性糖及其预处理后的回收率

生物质的化学成分，尤其是碳水化合物的含量，是生物质来源用于生物燃料的潜力的关键指标。这项研究的特点是从16个代表性的纯高粱突变体系的茎的理化特性。这项研究的目的是评估在木质素纤维素生物质通常使用的稀硫酸预处理条件下蔗糖及其水解产物，葡萄糖和果糖的回收率，并确定提取后的水提取物含量与糖回收率之间的关系。稀酸预处理的高粱秸秆对所有处理过的样品的糖化酶解糖化率均> 82.4％，纤维素回收率超过82.3％，半纤维素去除率达到85％。一个步骤，w / w）以降低加工成本并最大程度地减少废水处理，因为大多数糖将在稀酸预处理后以最少的降解产物回收。但是，对于高吸水率（> 35％，w / w）的高粱突变体茎，在进行预处理之前，预洗步骤有利于回收水溶性糖，以避免糖在加工过程中损失。预处理阶段。因此，应将不同的加工技术应用于具有各种水提取物含量和水溶性糖浓度的木质纤维素生物质。