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Occurrence of the genes encoding carbapenemases, ESBLs and class 1 integron‐integrase among fermenting and non‐fermenting bacteria from retail goat meat
Letters in Applied Microbiology ( IF 2.4 ) Pub Date : 2020-08-31 , DOI: 10.1111/lam.13368
F Singh 1, 2 , S D Hirpurkar 1 , N Rawat 1 , S Shakya 3 , R Kumar 4 , P K Rajput 4 , S Kumar 4
Affiliation  

The present study was planned to detect the genes encoding carbapenemases, ESBLs and class 1 integron‐integrase among bacteria obtained from retail goat meat. Fermenting and non‐fermenting bacterial isolates (n = 57), recovered from 61 goat meat samples, were identified by 16S rRNA gene sequencing. Antimicrobial susceptibility of isolates was tested by the broth dilution method using ceftazidime, cefotaxime, meropenem and imipenem. Plasmids were isolated and tested for their physical characters. Plasmids were subjected to screening of carbapenemase, ESBL and intI1 gene. Conjugation assay was performed using blaNDM‐positive isolates as the donor, and Escherichia coli HB101 as the recipient. Isolates showed the high rates of resistance to ceftazidime (77·2%), cefotaxime (70·2%), meropenem (22·8%) and imipenem (17·5%). They showed variability in number and size (~1 to >20 kb) of plasmids. Among all, 1, 4, 13 and 31 isolates showed the blaKPC, blaNDM, blaSHV and blaTEM genes, respectively. The blaKPC‐2 gene was observed in one E. coli isolate. The blaNDM‐1 gene was detected in Stenotrophomonas maltophilia (n = 2), Acinetobacter baumannii (n = 1) and Ochrobactrum anthropi (n = 1) isolates. These isolates co‐harboured the blaTEM and blaSHV genes. The intI1 gene was detected in 22 (38·6%) isolates, and 16 of these isolates showed the carbapenemase and/or ESBL genes. The conjugative movement of blaNDM gene could not be proved after three repetitive mating experiments. The presence of genes encoding carbapenemases and ESBLs in bacteria from goat meat poses public health risks.

中文翻译:

零售山羊肉发酵和非发酵细菌中碳青霉烯酶、ESBLs 和 1 类整合子整合酶基因的出现

本研究计划检测从零售山羊肉中获得的细菌中编码碳青霉烯酶、ESBLs 和 1 类整合子整合酶的基因。通过 16S rRNA 基因测序鉴定了从 61 个山羊肉样品中回收的发酵和非发酵细菌分离物(n = 57)。使用头孢他啶、头孢噻肟、美罗培南和亚胺培南通过肉汤稀释法测试分离株的抗菌敏感性。分离质粒并测试它们的物理特性。对质粒进行碳青霉烯酶、ESBL和intI1基因的筛选。使用blaNDM阳性分离株作为供体,大肠杆菌HB101作为受体进行结合测定。分离株对头孢他啶 (77·2%)、头孢噻肟 (70·2%)、美罗培南 (22·8%) 和亚胺培南 (17·5%) 的耐药率很高。他们显示质粒的数量和大小(~1 至 >20 kb)存在差异。其中,1、4、13 和 31 个分离株分别显示了 blaKPC、blaNDM、blaSHV 和 blaTEM 基因。在一种大肠杆菌分离物中观察到 blaKPC-2 基因。blaNDM-1 基因在嗜麦芽窄食单胞菌 (n = 2)、鲍曼不动杆菌 (n = 1) 和 Ochrobactrum anthropi (n = 1) 分离株中检测到。这些分离株共同携带了 blaTEM 和 blaSHV 基因。在22株(38·6%)分离株中检测到intI1基因,其中16株显示出碳青霉烯酶和/或ESBL基因。blaNDM基因的接合运动经过3次重复交配实验均未得到证实。来自山羊肉的细菌中存在编码碳青霉烯酶和 ESBLs 的基因会带来公共健康风险。13 和 31 株分别显示 blaKPC、blaNDM、blaSHV 和 blaTEM 基因。在一种大肠杆菌分离物中观察到 blaKPC-2 基因。blaNDM-1 基因在嗜麦芽窄食单胞菌 (n = 2)、鲍曼不动杆菌 (n = 1) 和 Ochrobactrum anthropi (n = 1) 分离株中检测到。这些分离株共同携带了 blaTEM 和 blaSHV 基因。在22株(38·6%)分离株中检测到intI1基因,其中16株显示出碳青霉烯酶和/或ESBL基因。blaNDM基因的接合运动经过3次重复交配实验均未得到证实。来自山羊肉的细菌中存在编码碳青霉烯酶和 ESBLs 的基因会带来公共健康风险。13 和 31 株分别显示 blaKPC、blaNDM、blaSHV 和 blaTEM 基因。在一种大肠杆菌分离物中观察到 blaKPC-2 基因。blaNDM-1 基因在嗜麦芽窄食单胞菌 (n = 2)、鲍曼不动杆菌 (n = 1) 和 Ochrobactrum anthropi (n = 1) 分离株中检测到。这些分离株共同携带了 blaTEM 和 blaSHV 基因。在22株(38·6%)分离株中检测到intI1基因,其中16株显示出碳青霉烯酶和/或ESBL基因。blaNDM基因的接合运动经过3次重复交配实验均未得到证实。来自山羊肉的细菌中存在编码碳青霉烯酶和 ESBLs 的基因会带来公共健康风险。鲍曼不动杆菌 (n = 1) 和 Ochrobactrum anthropi (n = 1) 分离株。这些分离物共同携带了 blaTEM 和 blaSHV 基因。在22株(38·6%)分离株中检测到intI1基因,其中16株显示出碳青霉烯酶和/或ESBL基因。blaNDM基因的接合运动经过3次重复交配实验均未得到证实。来自山羊肉的细菌中存在编码碳青霉烯酶和 ESBLs 的基因会带来公共健康风险。鲍曼不动杆菌 (n = 1) 和 Ochrobactrum anthropi (n = 1) 分离株。这些分离株共同携带了 blaTEM 和 blaSHV 基因。在22株(38·6%)分离株中检测到intI1基因,其中16株显示出碳青霉烯酶和/或ESBL基因。blaNDM基因的接合运动经过3次重复交配实验均未得到证实。来自山羊肉的细菌中存在编码碳青霉烯酶和 ESBLs 的基因会带来公共健康风险。
更新日期:2020-08-31
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