Mass spectrometry imaging (MSI) allows investigating the spatial distribution of chemical compounds directly in biological tissues. As the analytical depth of MSI is limited, MSI needs to be coupled to more sensitive local extraction‐based omics approaches to achieve a comprehensive molecular characterization. For this, it is important to retain the spatial information provided by MSI for follow‐up omics studies. It has been shown that regiospecific MSI data can be used to guide a laser microdissection system for ultra‐sensitive liquid chromatography‐mass spectrometry (LC‐MS) analyses. So far, this combination has required separate and specialized mass spectrometry (MS) instrumentation. Recent advances in dual‐source instrumentation, harboring both matrix assisted laser/desorption ionization (MALDI) and electrospray ionization (ESI) sources, promise state‐of‐the‐art MSI and liquid‐based proteomic capabilities on the same MS instrument. This study demonstrates that such an instrument can offer both fast lipid‐based MSI at high mass and high lateral resolution and sensitive LC‐MS on local protein extracts from the exact same tissue section.

中文翻译：

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单台仪器上空间组学的 MS 成像引导微蛋白质组学。

质谱成像 (MSI) 允许直接研究生物组织中化合物的空间分布。由于 MSI 的分析深度有限，MSI 需要与更灵敏的基于局部提取的组学方法相结合，以实现全面的分子表征。为此，保留 MSI 提供的空间信息用于后续组学研究非常重要。已经表明，区域特异性 MSI 数据可用于指导激光显微切割系统进行超灵敏液相色谱-质谱 (LC-MS) 分析。到目前为止，这种组合需要单独和专门的质谱 (MS) 仪器。双源仪器的最新进展，包括基质辅助激光/解吸电离 (MALDI) 和电喷雾电离 (ESI) 源，承诺在同一台 MS 仪器上提供最先进的 MSI 和基于液体的蛋白质组学功能。该研究表明，这种仪器可以提供高质量和高横向分辨率的基于脂质的快速 MSI，以及对来自完全相同组织切片的局部蛋白质提取物的灵敏 LC-MS。