The rhizosphere microbiome plays an important role in the growth and health of many plants, particularly for plant growth‐promoting rhizobacteria (PGPR). Although the use of PGPR could improve plant production, real‐world applications are still held back by low‐efficiency methods of finding and using PGPR. In this study, the structure of bacterial and fungal rhizosphere communities of Jinxiang garlic under different growth periods (resume growth, bolting and maturation), soil types (loam, sandy loam and sandy soil) and agricultural practices (with and without microbial products) were explored by using amplicon sequencing. High‐efficiency top‐down approaches based on high‐throughput technology and synthetic community (SynCom) approaches were used to find PGPR in garlic rhizosphere and improve plant production. Our findings indicated that Pseudomonas was a key PGPR in the rhizosphere of garlic. Furthermore, SynCom with six Pseudomonas strains isolated from the garlic rhizosphere were constructed, which showed that they have the ability to promote plant growth.

中文翻译：

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从大蒜根际微生物群中筛选出6个假单胞菌菌株的合成群落可促进植物生长

根际微生物组在许多植物的生长和健康中起着重要作用，特别是对于促进植物生长的根际细菌（PGPR）。尽管使用PGPR可以提高植物的产量，但发现和使用PGPR的低效率方法仍然阻碍了实际应用。在这项研究中，金乡大蒜在不同的生长期（恢复生长，抽，和成熟），土壤类型（壤土，砂壤土和沙质土壤）和农业实践（有或没有微生物产品）下的细菌和真菌的根际群落结构均为通过使用扩增子测序探索。使用基于高通量技术和合成社区（SynCom）方法的高效自上而下的方法来发现大蒜根际中的PGPR，并提高植物产量。我们的发现表明假单胞菌是大蒜根际中的关键PGPR。此外，构建了具有从大蒜根际分离出的6个假单胞菌菌株的SynCom ，这表明它们具有促进植物生长的能力。