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Effects of methylparaben on in vitro maturation of porcine oocytes.
Journal of Applied Toxicology ( IF 3.3 ) Pub Date : 2020-08-07 , DOI: 10.1002/jat.4045
Adyeni Barajas-Salinas 1, 2 , Yvonne Ducolomb 2 , Miguel Betancourt 2 , Elivier Núñez-Macías 2 , Alma López 2 , Jaime Barraza 2 , Juan Quezadas-Fuentes 3 , Iván Bahena-Ocampo 2 , Edmundo Bonilla 2 , Socorro Retana-Márquez 3 , Eduardo Casas 2 , Fahiel Casillas 3
Affiliation  

Parabens (PBs) are compounds widely used in industry for food and personal care products as antimicrobials and preservatives. Their indiscriminate use has resulted in their detection in different ecosystems so that humans and other organisms are highly exposed. Methylparaben (MePB), compared with other PBs, is mostly detected in food, personal care and baby care products. PBs could be linked to the generation of hormonal disorders and fertility impairment since their recent classification as endocrine disruptors. The knowledge of the effects that MePB can exert is of great importance as, in terms of reproduction, information is limited. Therefore, the objective of the present study was to evaluate the effect of MePB on porcine oocyte viability and in vitro maturation (IVM), as well as to determine the lethal concentration at 50% (LC50) and the maturation inhibition concentration at 50% (MIC50). Oocytes were exposed to different MePB concentrations 0 (control), 50, 100, 500, 750 and 1000 μm during 44 h of IVM. Cytoplasmic alterations and reduced cumulus cell expansion were observed in oocytes exposed to MePB; however, viability was not affected. In addition, oocyte maturation decreased in a concentration‐dependent manner after exposure to MePB. The estimated LC50 was 2028.38 μm, whereas MIC50 was 780.31 μm. To our knowledge, this is the first study that demonstrates that MePB altered porcine oocyte morphology, and caused a reduction in cumulus cell expansion, both of which resulted in decreased oocyte maturation. Therefore, MePB exposure may be one of the factors involved in fertility impairment in mammals, including that of humans.

中文翻译:

对羟基苯甲酸甲酯对猪卵母细胞体外成熟的影响。

对羟基苯甲酸酯 (PB) 是广泛用于食品和个人护理产品工业中作为抗菌剂和防腐剂的化合物。它们的滥用导致它们在不同的生态系统中被发现,因此人类和其他生物高度暴露。与其他 PB 相比,对羟基苯甲酸甲酯 (MePB) 主要存在于食品、个人护理和婴儿护理产品中。PBs 可能与荷尔蒙失调和生育障碍的产生有关,因为它们最近被归类为内分泌干扰物。了解 MePB 可以发挥的作用非常重要,因为就繁殖而言,信息是有限的。因此,本研究的目的是评估 MePB 对猪卵母细胞活力和体外成熟 (IVM) 的影响,以及确定 50% (LC50 ) 和 50% 的成熟抑制浓度 (MIC 50 )。卵母细胞暴露于不同浓度的市环保局0(对照),50,100,500,750和1000μIVM的44小时期间。在暴露于 MePB 的卵母细胞中观察到细胞质改变和卵丘细胞扩增减少;然而,生存能力没有受到影响。此外,暴露于 MePB 后,卵母细胞成熟度呈浓度依赖性下降。估计的 LC 50为 2028.38 μ m,而 MIC 50为 780.31 μ m. 据我们所知,这是第一项证明 MePB 改变猪卵母细胞形态并导致卵丘细胞扩张减少的研究,这两者都导致卵母细胞成熟减少。因此,MePB 暴露可能是导致哺乳动物(包括人类)生育能力受损的因素之一。
更新日期:2020-08-07
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