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Efficient bacterial isolate from roots of cactus degrading Reactive Black 5
Environmental Technology & Innovation ( IF 7.1 ) Pub Date : 2020-08-07 , DOI: 10.1016/j.eti.2020.101082
Asima Bibi , Hong-xiang Zhu , Qaisar Mahmood , Jin Wang , Xu-Dong Li , Mujaddad-ur-Rehman , Tahir Hayat , Nusrat Shaheen , Arshad Ali

The current study aimed at azo dye degradation using a facultative anaerobe. A bacterial strain capable of decolorization and degradation of RB5 under static conditions was isolated from root nodules of Cactus. The strain efficiently degraded Reactive Black 5 (RB5) with metabolites detected by GC–MS. The strain was identified as Proteus mirabilis and placed in phylogenetic position through 16S rRNA gene analysis. The degradation increased with time to achieve up to 90 % after 72 h incubation at 37 ºC and pH value of 7. Variations of C source did not affect the process while yeast extract was effective. The effective degradation was achieved at 100 ppm of dye. The GC–MS detected metabolites included; acetaldoxime, oxirane trimethyl, acetic acid-2-propenyl ester, acetaldehyde semi carbazone, 2-pentanone, 4-hydroxy-4-methyl, hydrazine, 1-5-hexenyl-1-methyl and enanthamide Protox and phytotoxicity results showed fewer toxic ranges of these metabolites as compared to products of benzene and amines. The isolated strain can be useful in the bioremediation of azo dyes contaminated environments.



中文翻译:

仙人掌根部有效细菌分离物降解活性黑5

当前的研究旨在使用兼性厌氧菌降解偶氮染料。从仙人掌的根瘤中分离出能够在静态条件下使RB5脱色和降解的细菌菌株。该菌株通过GC-MS检测到的代谢物有效降解了活性黑5(RB5)。该菌株被鉴定为奇异变形杆菌并通过16S rRNA基因分析将其置于系统发育位置。在37ºC和pH值为7的条件下孵育72小时后,降解随时间增加达到90%。当酵母提取物有效时,C源的变化不会影响该过程。在100 ppm的染料下可实现有效降解。GC-MS检测到包括代谢产物;乙醛肟,环氧乙烷三甲基,乙酸-2-丙烯酯,乙醛半碳巴酮,2-戊酮,4-羟基-4-甲基,肼,1-5-己烯基-1-甲基和庚酰胺原型及植物毒性结果显示,毒性范围较小这些代谢产物与苯和胺的产物相比。分离的菌株可用于对偶氮染料污染的环境进行生物修复。

更新日期:2020-08-29
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