Background

Parkinson's disease (PD) is a prevalent neurodegenerative illness. It has been believed that oxidative stress (OS) is an important factor in the advancement of PD. This investigation attempts to evaluate the relations between blood trace elements, ferritin, and transferrin concentrations as well as the levels of protein and gene expression of ceruloplasmin (CP), Nrf-2, and HO-1 in patients suffering PD.

Methods

The serum concentrations of variables were assessed in 110 PD patients group and 110 normal subjects. Furthermore, we applied qRT-PCR as well as western blot (WB) analysis to measure the levels of gene and protein, respectively.

Results

Considerable differences were detected in the serum concentrations of copper (Cu), iron (Fe), and zinc (Zn), when healthy and patient groups were compared. Nevertheless, the levels of Se, ferritin, and transferrin were not significantly different between the two groups. qRT-PCR and WB data analysis revealed significant differences of CP, Nrf-2, and HO-1at genes expression and protein levels when comparing the two PD patients and control groups.

Conclusion

The results of the current work revealed that blood levels of Cu, Fe, and Zn were significantly higher in subjects who had PD. In addition, it was found that the levels of protein and gene expression CP, Nrf-2, and HO-1 were markedly higher in PD group than in non-PD subjects. Indeed, in this study, the results showed that the antioxidant content of the body can be linked to PD.

中文翻译：

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人体抗氧化剂含量异常与帕金森氏病的发生和发展有关。

背景

帕金森氏病（PD）是一种普遍的神经退行性疾病。已经认为氧化应激（OS）是PD发展的重要因素。这项研究试图评估患PD的患者血液中微量元素，铁蛋白和转铁蛋白浓度之间的关系，以及铜蓝蛋白（CP），Nrf-2和HO-1的蛋白质和基因表达水平。

方法

在110名PD患者组和110名正常受试者中评估了血清浓度。此外，我们应用了qRT-PCR和Western blot（WB）分析来分别测量基因和蛋白质的水平。

结果

当比较健康组和患者组时，血清铜，铁，铁和锌的浓度差异显着。尽管如此，两组之间的硒，铁蛋白和转铁蛋白水平没有显着差异。比较两个PD患者和对照组，qRT-PCR和WB数据分析显示CP，Nrf-2和HO-1at基因表达和蛋白质水平存在显着差异。

结论

当前工作的结果表明，患有PD的受试者的血液中Cu，Fe和Zn的水平明显更高。另外，发现PD组的蛋白质和基因表达CP，Nrf-2和HO-1的水平显着高于非PD受试者。确实，在这项研究中，结果表明人体的抗氧化剂含量可以与PD关联。