Advances in Chromatin and Chromosome Research: Perspectives from Multiple Fields.,Molecular Cell

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Advances in Chromatin and Chromosome Research: Perspectives from Multiple Fields.
Molecular Cell ( IF 16.0 ) Pub Date : 2020-08-07 , DOI: 10.1016/j.molcel.2020.07.003
Andrews Akwasi Agbleke ₁ , Assaf Amitai ₂ , Jason D Buenrostro ₃ , Aditi Chakrabarti ₄ , Lingluo Chu ₅ , Anders S Hansen ₆ , Kristen M Koenig ₇ , Ajay S Labade ₃ , Sirui Liu ₈ , Tadasu Nozaki ₅ , Sergey Ovchinnikov ₉ , Andrew Seeber ₁₀ , Haitham A Shaban ₁₁ , Jan-Hendrik Spille ₁₂ , Andrew D Stephens ₁₃ , Jun-Han Su ₅ , Dushan Wadduwage ₁₀

Affiliation

Center for Advanced Imaging, Harvard University, Cambridge, MA 02138, USA.
Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA; JHDSF Program, Harvard University, Cambridge, MA 02138, USA.
FAS Division of Science, Harvard University, Cambridge, MA 02138, USA.
JHDSF Program, Harvard University, Cambridge, MA 02138, USA; FAS Division of Science, Harvard University, Cambridge, MA 02138, USA.
JHDSF Program, Harvard University, Cambridge, MA 02138, USA; Center for Advanced Imaging, Harvard University, Cambridge, MA 02138, USA.
Center for Advanced Imaging, Harvard University, Cambridge, MA 02138, USA; Spectroscopy Department, Physics Division, National Research Centre, Dokki, 12622 Cairo, Egypt.
Department of Physics, University of Illinois at Chicago, Chicago, IL 60607, USA.
Biology Department, University of Massachusetts, Amherst, Amherst, MA 01003, USA.

Nucleosomes package genomic DNA into chromatin. By regulating DNA access for transcription, replication, DNA repair, and epigenetic modification, chromatin forms the nexus of most nuclear processes. In addition, dynamic organization of chromatin underlies both regulation of gene expression and evolution of chromosomes into individualized sister objects, which can segregate cleanly to different daughter cells at anaphase. This collaborative review shines a spotlight on technologies that will be crucial to interrogate key questions in chromatin and chromosome biology including state-of-the-art microscopy techniques, tools to physically manipulate chromatin, single-cell methods to measure chromatin accessibility, computational imaging with neural networks and analytical tools to interpret chromatin structure and dynamics. In addition, this review provides perspectives on how these tools can be applied to specific research fields such as genome stability and developmental biology and to test concepts such as phase separation of chromatin.

中文翻译：

染色质和染色体研究进展：多领域视角。

核小体将基因组 DNA 包装到染色质中。通过调节转录、复制、DNA 修复和表观遗传修饰的 DNA 通路，染色质形成了大多数核过程的纽带。此外，染色质的动态组织是基因表达调控和染色体进化成个体化姐妹对象的基础，这些对象可以在后期完全分离到不同的子细胞。这篇合作评论聚焦于对询问染色质和染色体生物学关键问题至关重要的技术，包括最先进的显微镜技术、物理操作染色质的工具、测量染色质可及性的单细胞方法、计算成像神经网络和分析工具来解释染色质结构和动力学。此外，

更新日期：2020-09-18

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